2021年6月17日，《自然—遗传学》杂志在线发表了美国索尔克生物研究所Joanne Chory、Joseph R. Ecker等研究人员的最新成果。该研究发现，植物光敏素相互作用因子可触发植物中的环境响应染色质动力学。

研究人员表示，光受体和光敏色素相互作用因子（PIF）之间的相互作用是一个调节中心，可感知环境线索并将其整合到植物的转录网络中。尽管组蛋白变体H2A.Z的占据和组蛋白H3的乙酰化已成为环境响应基因网络的调节因子，但这些表观基因组特征如何与PIF活性相互作用尚不清楚。

通过利用快速且可逆的光介导PIF7亚核定位和磷酸化操作，研究人员同时测定了PIF7的DNA结合特性，以及它对全基因组染色质动力学的影响。研究人员发现PIF会迅速行动以响应光质量变化来重塑H2A.Z和H3K9ac表观遗传图谱。

此外，研究人员发现PIF通过与染色质重塑复合物亚基INO80亚基6（Ies6）的拟南芥同系物EIN6 ENHANCER（EEN）直接相互作用实现H2A.Z的移除。因此，研究人员描述了一个PIF-INO80调节模块，它能够使得植物改变其生长轨迹从而响应环境变化。 

附：英文原文

Title: PHYTOCHROME-INTERACTING FACTORs trigger environmentally responsive chromatin dynamics in plants

Author: Bjrn C. Willige, Mark Zander, Chan Yul Yoo, Amy Phan, Renee M. Garza, Shelly A. Trigg, Yupeng He, Joseph R. Nery, Huaming Chen, Meng Chen, Joseph R. Ecker, Joanne Chory

Issue&Volume: 2021-06-17

Abstract: The interplay between light receptors and PHYTOCHROME-INTERACTING FACTORs (PIFs) serves as a regulatory hub that perceives and integrates environmental cues into transcriptional networks of plants1,2. Although occupancy of the histone variant H2A.Z and acetylation of histone H3 have emerged as regulators of environmentally responsive gene networks, how these epigenomic features interface with PIF activity is poorly understood3,4,5,6,7. By taking advantage of rapid and reversible light-mediated manipulation of PIF7 subnuclear localization and phosphorylation, we simultaneously assayed the DNA-binding properties of PIF7, as well as its impact on chromatin dynamics genome wide. We found that PIFs act rapidly to reshape the H2A.Z and H3K9ac epigenetic landscape in response to a change in light quality. Furthermore, we discovered that PIFs achieve H2A.Z removal through direct interaction with EIN6 ENHANCER (EEN), the Arabidopsis thaliana homolog of the chromatin remodeling complex subunit INO80 Subunit 6 (Ies6). Thus, we describe a PIF–INO80 regulatory module that is an intermediate step for allowing plants to change their growth trajectory in response to environmental changes.

DOI: 10.1038/s41588-021-00882-3

Source: https://www.nature.com/articles/s41588-021-00882-3