为了解码在发育过程中调节纹状体的转录模块，研究人员完成了从头鉴定出的1116个长基因间非编码RNA（lincRNA）的目录，然后从早期人类胎儿纹状体分析了96789个单细胞。研究人员发现，D1和D2中型棘突神经元（D1-和D2-MSNs）来自一个共同的祖细胞，并且在有丝分裂后的过渡过程中，谱系得以建立。然后，研究人员发现了通过计算机干扰验证的特定于细胞类型的基因调控网络。
最后，研究人员鉴定了人类特异性的lincRNA，它们在人类中促进了该结构的系统发育差异。这项工作描绘了控制MSN谱系建立的细胞层级。
据悉，理解人类纹状体的发育是了解影响该区域疾病的必要条件。
附：英文原文
Title: The coding and long noncoding single-cell atlas of the developing human fetal striatum
Author: Vittoria Dickinson Bocchi, Paola Conforti, Elena Vezzoli, Dario Besusso, Claudio Cappadona, Tiziana Lischetti, Maura Galimberti, Valeria Ranzani, Raoul J. P. Bonnal, Marco De Simone, Grazisa Rossetti, Xiaoling He, Kenji Kamimoto, Ira Espuny-Camacho, Andrea Faedo, Federica Gervasoni, Romina Vuono, Samantha A. Morris, Jian Chen, Dan Felsenfeld, Giulio Pavesi, Roger A. Barker, Massimiliano Pagani, Elena Cattaneo
Issue&Volume: 2021/05/07
Abstract: Deciphering how the human striatum develops is necessary for understanding the diseases that affect this region. To decode the transcriptional modules that regulate this structure during development, we compiled a catalog of 1116 long intergenic noncoding RNAs (lincRNAs) identified de novo and then profiled 96,789 single cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (D1- and D2-MSNs) arise from a common progenitor and that lineage commitment is established during the postmitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type–specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. This work delineates the cellular hierarchies governing MSN lineage commitment.
DOI: 10.1126/science.abf5759
Source: https://science.sciencemag.org/content/372/6542/eabf5759