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活胚胎中翻译动力学揭示发育的空间异质性
作者:小柯机器人 发布时间:2021/4/30 18:13:09

法国蒙彼利埃大学Mounia Lagha研究组利用活体胚胎中的成像翻译动力学揭示了空间异质性。该研究于2021年4月29日发表于国际一流学术期刊《科学》杂志上。

他们建立了SunTag方法以可视化和量化果蝇胚胎中单个mRNA的翻译的时间、位置和动力学。通过集中于保守的主要上皮-间质转化(EMT)诱导转录因子Twist的翻译,他们确定了mRNA翻译效率的空间异质性并揭示了翻译工厂的存在,其中簇化的mRNAs优先在基底周核区共翻译。观察活多细胞生物中mRNA翻译的位置和动力学为理解发育过程中的基因调控开辟了途径。

据介绍,关于将mRNA翻译成蛋白质所涉及的因素,人们已经了解了很多。但是,这种多步骤过程尚未在活的多细胞生物中成像。

附:英文原文

Title: Imaging translation dynamics in live embryos reveals spatial heterogeneities

Author: Jeremy Dufourt, Maelle Bellec, Antonio Trullo, Matthieu Dejean, Sylvain De Rossi, Cyril Favard, Mounia Lagha

Issue&Volume: 2021/04/29

Abstract: Much is known about the factors involved in the translation of mRNA into protein; however, this multi-step process has not been imaged in living multicellular organisms. Here, we deploy the SunTag method to visualize and quantify the timing, location, and kinetics of the translation of single mRNAs in living Drosophila embryos. By focusing on the translation of the conserved major epithelial-mesenchymal transition (EMT)-inducing transcription factor Twist, we identified spatial heterogeneity in mRNA translation efficiency and reveal the existence of translation factories, where clustered mRNAs are co-translated preferentially at basal perinuclear regions. Observing the location and dynamics of mRNA translation in a living multicellular organism opens avenues for understanding gene regulation during development.

DOI: 10.1126/science.abc3483

Source: https://science.sciencemag.org/content/early/2021/04/28/science.abc3483

期刊信息
Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037