美国匹兹堡大学Mark J. Shlomchik小组揭示小鼠和人体组织中初始和记忆B细胞的表面表型。相关论文于2021年12月22日在线发表在《自然—免疫学》杂志上。

研究人员表示，记忆B细胞（MBC）保护身体免受反复感染。MBC在许多方面与其初始对应物（NBC）不同，但功能和表面标志物的差异没有得到很好的描述。此外，尽管小鼠是人类免疫学的普遍模型，但有关B细胞区室同源性的信息是有限的。

为了解决这个问题，研究人员对人类和小鼠的MBC进行了无偏倚的大规模筛选，以确定其表面标志物的不同表达。通过将这些标记物的表达与高维流式细胞仪中的大量已知标记物相关联，研究人员全面地确定了MBC和NBC之间差异表达的许多表面蛋白。这些标记物的组合使研究人员能够识别人类和小鼠的MBC，并深入了解它们的功能差异。这些结果将大大加强对体液免疫的理解，并可用于改善免疫监测。

附：英文原文

Title: Surface phenotypes of naive and memory B cells in mouse and human tissues

Author: Weisel, Nadine M., Joachim, Stephen M., Smita, Shuchi, Callahan, Derrick, Elsner, Rebecca A., Conter, Laura J., Chikina, Maria, Farber, Donna L., Weisel, Florian J., Shlomchik, Mark J.

Issue&Volume: 2021-12-22

Abstract: Memory B cells (MBCs) protect the body from recurring infections. MBCs differ from their naive counterparts (NBCs) in many ways, but functional and surface marker differences are poorly characterized. In addition, although mice are the prevalent model for human immunology, information is limited concerning the nature of homology in B cell compartments. To address this, we undertook an unbiased, large-scale screening of both human and mouse MBCs for their differential expression of surface markers. By correlating the expression of such markers with extensive panels of known markers in high-dimensional flow cytometry, we comprehensively identified numerous surface proteins that are differentially expressed between MBCs and NBCs. The combination of these markers allows for the identification of MBCs in humans and mice and provides insight into their functional differences. These results will greatly enhance understanding of humoral immunity and can be used to improve immune monitoring.

DOI: 10.1038/s41590-021-01078-x

Source: https://www.nature.com/articles/s41590-021-01078-x