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研究完成小鼠大脑中谱系和遗传特性的单细胞图谱
作者:小柯机器人 发布时间:2021/12/19 23:51:40

2021年12月15日,《自然》杂志在线发表了德国马克斯·普朗克神经生物学研究所Christian Mayer团队的最新成果。该研究完成了小鼠大脑中谱系和遗传特性的单细胞图谱。

研究人员使用大规模平行标记的祖先来跟踪了小鼠前脑发育过程中的克隆关系和转录组特征。研究人员量化了出生后所有主要细胞类别的克隆分化和趋同,并发现不同类型的GABA能神经元有一个共同的谱系。GABA能克隆的分化发生在胚胎发育过程中的细胞周期退出时,这表明亚型的分化是在祖细胞水平上作为一个依赖系谱的过程开始的。

据了解,在神经发生过程中,小鼠胚胎脑室内的有丝分裂祖细胞经历了最后一轮的细胞分裂,产生了广泛的有丝分裂后的神经元和胶质细胞。发育谱系和细胞类型多样性之间的联系仍然是一个开放的问题。

附:英文原文

Title: Single-cell delineation of lineage and genetic identity in the mouse brain

Author: Bandler, Rachel C., Vitali, Ilaria, Delgado, Ryan N., Ho, May C., Dvoretskova, Elena, Ibarra Molinas, Josue S., Frazel, Paul W., Mohammadkhani, Maesoumeh, Machold, Robert, Maedler, Sophia, Liddelow, Shane A., Nowakowski, Tomasz J., Fishell, Gord, Mayer, Christian

Issue&Volume: 2021-12-15

Abstract: During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and cell-type diversity remains an open question. Here we used massively parallel tagging of progenitors to track clonal relationships and transcriptomic signatures during mouse forebrain development. We quantified clonal divergence and convergence across all major cell classes postnatally, and found diverse types of GABAergic neuron that share a common lineage. Divergence of GABAergic clones occurred during embryogenesis upon cell-cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor cell level. Single-cell RNA sequencing with parallel tagging of progenitor cells is used to track clonal relationships and transcriptomic signatures during development of the mouse forebrain.

DOI: 10.1038/s41586-021-04237-0

Source: https://www.nature.com/articles/s41586-021-04237-0

期刊信息

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html