美国普渡大学Chengde Mao研究组实现动态的DNA自组装。该项研究成果于2021年11月22日在线发表在《美国化学会杂志》上。

研究人员表示，DNA折纸是一种构建DNA纳米结构的强大方法。它需要长的单链DNA。这种长的DNA链的制备往往是相当繁琐的，而且产量有限。相比之下，双链DNA可以很容易地通过酶促反应大量制备。

因此，研究人员提出了一个问题：能否以这样的方式设计DNA纳米结构，使两条互补链能在同一溶液中同时折叠成设计的结构，而不是相互杂交形成DNA双链？通过工程化的DNA相互作用动力学，研究人员提供了多个例子来回答了这个问题。产生的DNA纳米结构通过电泳和原子力显微镜成像进行了彻底的表征。

该研究报告的策略与DNA克隆方法兼容，因此将为DNA纳米结构的大规模生产提供一个简便的方法。

附：英文原文

Title: Kinetic DNA Self-Assembly: Simultaneously Co-folding Complementary DNA Strands into Identical Nanostructures

Author: Mengxi Zheng, Zhe Li, Longfei Liu, Mo Li, Victoria E. Paluzzi, Jong Hyun Choi, Chengde Mao

Issue&Volume: November 22, 2021

Abstract: DNA origami is a powerful method for constructing DNA nanostructures. It requires long single-stranded DNAs. The preparation of such long DNA strands is often quite tedious and has a limited production yield. In contrast, duplex DNAs can be easily prepared via enzymatic reactions in large quantities. Thus, we ask a question: can we design DNA nanostructures in such a way that the two complementary strands can simultaneously fold into the designed structures in the same solution instead of hybridizing with each other to form a DNA duplex By engineering DNA interaction kinetics, herein we are able to provide multiple examples to concretely demonstrate a positive answer to this question. The resulting DNA nanostructures have been thoroughly characterized by electrophoresis and atomic force microscopy imaging. The reported strategy is compatible with the DNA cloning method and thus would provide a convenient method for the large-scale production of the designed DNA nanostructures.

DOI: 10.1021/jacs.1c09925

Source: https://pubs.acs.org/doi/10.1021/jacs.1c09925