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巨噬细胞亚群为细胞内沙门氏菌提供了脾脏的复制微环境
作者:小柯机器人 发布时间:2021/11/22 13:27:30

以色列魏茨曼科学研究所Roi Avraham团队发现,非经典单核细胞衍生的巨噬细胞亚群为细胞内沙门氏菌提供了脾脏的复制微环境。相关论文于2021年11月16日在线发表在《免疫》杂志上。

研究人员调查了小鼠早期系统性感染期间细胞内伤寒沙门氏菌(S.Tm)的单核微环境。研究人员描述了脾脏中日蚀式的生长动力学,第一阶段的细菌控制是由组织内的红浆巨噬细胞介导的。第二阶段是在以CD9表达为特征的巨噬细胞群中进行广泛的细菌复制。研究人员证明,CD9+巨噬细胞诱导了解毒脂质的途径,这可能被细胞内的S.Tm利用。研究人员确定CD9+巨噬细胞起源于非经典单核细胞(NCM),而且NCM耗尽的小鼠对S.Tm感染更有抵抗力。这项研究确定了巨噬细胞亚群特异性的宿主-病原体相互作用,从而决定整个机体的早期感染动态和感染结果。

据悉,细胞内细菌和单核吞噬细胞之间的相互作用产生了不同的细胞表型,可能决定了感染的结果。最近单细胞RNA测序(scRNA-seq)的进展已经确定了单核群体中的多个亚群,但对其在感染期间的功能影响有限。

附:英文原文

Title: A non-classical monocyte-derived macrophage subset provides a splenic replication niche for intracellular Salmonella

Author: Dotan Hoffman, Yaara Tevet, Sébastien Trzebanski, Gili Rosenberg, Leia Vainman, Aryeh Solomon, Shelly Hen-Avivi, Noa Bossel Ben-Moshe, Roi Avraham

Issue&Volume: 2021-11-16

Abstract: Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA sequencing (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection are limited. Here, we surveyed the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We described eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a macrophage population characterized by CD9 expression. We demonstrated that CD9+ macrophages induced pathways for detoxificating oxidized lipids, that may be utilized by intracellular S.Tm. We established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines macrophage subset-specific host-pathogen interactions that determine early infection dynamics and infection outcome of the entire organism.

DOI: 10.1016/j.immuni.2021.10.015

Source: https://www.cell.com/immunity/fulltext/S1074-7613(21)00452-0

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx