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人原肠胚发育过程的单细胞转录组学特征获解析
作者:小柯机器人 发布时间:2021/11/22 12:48:49

英国牛津大学Shankar Srinivas和德国环境健康研究中心Antonio Scialdone研究组合作揭示了人原肠胚形成过程的单细胞转录组学特征。相关论文于2021年11月17日发表于国际学术期刊《自然》杂志。

研究人员以空间解析的方式表征了人胚胎受精后16到19天时整个原肠胚形成的单细胞转录谱。研究人员使用这些数据分析了原肠胚的细胞类型并与其他模型系统进行了比较。除了多能外胚层,研究还鉴定到原始生殖细胞、红细胞和各种中胚层和内胚层细胞类型。该数据集有助于人们理解发育过程中重要但未知阶段的特征。这种表征为解释其他模型系统中的实验数据提供了新的依据,这为体外人类细胞定向分化提供了宝贵的资源。

研究人员表示,原肠胚形成是所有多细胞动物发育的基础,这是个体发育的起始。它对细胞多样性与个体发育都至关重要。在人类中,原肠胚形成发生在受精后的第三周。但对人原肠胚形成过程的理解相对有限,主要来源于历史标本实验模型或最近的体外培养样品。

附:英文原文

Title: Single-cell transcriptomic characterization of a gastrulating human embryo

Author: Tyser, Richard C. V., Mahammadov, Elmir, Nakanoh, Shota, Vallier, Ludovic, Scialdone, Antonio, Srinivas, Shankar

Issue&Volume: 2021-11-17

Abstract: Gastrulation is the fundamental process in all multicellular animals through which the basic body plan is first laid down1,2,3,4. It is pivotal in generating cellular diversity coordinated with spatial patterning. In humans, gastrulation occurs in the third week after fertilization. Our understanding of this process in humans is relatively limited and based primarily on historical specimens5,6,7,8, experimental models9,10,11,12 or, more recently, in vitro cultured samples13,14,15,16. Here we characterize in a spatially resolved manner the single-cell transcriptional profile of an entire gastrulating human embryo, staged to be between 16 and 19 days after fertilization. We use these data to analyse the cell types present and to make comparisons with other model systems. In addition to pluripotent epiblast, we identified primordial germ cells, red blood cells and various mesodermal and endodermal cell types. This dataset offers a unique glimpse into a central but inaccessible stage of our development. This characterization provides new context for interpreting experiments in other model systems and represents a valuable resource for guiding directed differentiation of human cells in vitro.

DOI: 10.1038/s41586-021-04158-y

Source: https://www.nature.com/articles/s41586-021-04158-y

期刊信息

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html