高效分离高纯度和低细胞损伤的免疫细胞对于免疫治疗非常重要,并且仍然具有高度挑战性。
研究人员在该文中报告一种含有多价多模体的细胞捕获DNA网络,用于T淋巴细胞(T细胞)的特异性分离和原位培养。通过酶促扩增过程合成的两条超长DNA链被合理设计,包括作为细胞锚定物和免疫佐剂的功能性多模体。互补序列促进了DNA网络的形成和T细胞的包封,并为T细胞和免疫佐剂的反应性释放提供了限制性内切酶的切割位点。捕获的肿瘤浸润性T细胞纯度达98%,存活率维持在90%左右。含T细胞的DNA网络进一步用于肿瘤病灶的局部免疫治疗。
研究工作为有效分离免疫细胞和其他生物颗粒提供了强大的纳米生物技术。
附:英文原文
Title: T Lymphocyte-Captured DNA Network for Localized Immunotherapy
Author: Chi Yao, Chenxu Zhu, Jianpu Tang, Junhan Ou, Rui Zhang, Dayong Yang
Issue&Volume: November 15, 2021
Abstract: The efficient isolation of immune cells with high purity and low cell damage is important for immunotherapy and remains highly challenging. We herein report a cell capture DNA network containing polyvalent multimodules for the specific isolation and in situ incubation of T lymphocytes (T-cells). Two ultralong DNA chains synthesized by an enzymatic amplification process were rationally designed to include functional multimodules as cell anchors and immune adjuvants. Mutually complementary sequences facilitated the formation of a DNA network and encapsulation of T-cells, as well as offering cutting sites of a restriction enzyme for the responsive release of T-cells and immune adjuvants. The purity of captured tumor-infiltrating T-cells reached 98%, and the viability of T-cells maintained ~90%. The T-cells-containing DNA network was further administrated to a tumor lesion for localized immunotherapy. Our work provides a robust nanobiotechnology for efficient isolation of immune cells and other biological particles.
DOI: 10.1021/jacs.1c07036
Source: https://pubs.acs.org/doi/10.1021/jacs.1c07036
JACS:《美国化学会志》,创刊于1879年。隶属于美国化学会,最新IF:14.612
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