美国马萨诸塞大学阿默斯特分校Mingxu You团队报道了DNA拉链探针成像活细胞中的膜顺序和动态相互作用。相关研究成果发表在2021年11月12日出版的《德国应用化学》。

细胞膜是由不同的子室组成的动态异质结构。在这些隔间内，各种脂质和蛋白质之间发生优先相互作用。目前，对这些短寿命膜复合物的成像仍然是一个挑战，尤其是在活细胞中。

该文中，研究人员提出了一种基于DNA的探针，称为“DNA拉链”，它能够使用标准荧光显微镜在活细胞中成像膜顺序和瞬时相互作用模式。通过微调DNA双链的长度和结合亲和力，该探针可以通过动态DNA杂交精确延长膜脂相互作用的持续时间。还研究了膜顺序与T细胞受体信号激活之间的相关性。该可编程的DNA探针在短暂的细胞培养后发挥作用，可以很容易地用于研究不同膜信号事件期间的脂质相互作用和膜顺序。

附：英文原文

Title: Imaging Membrane Order and Dynamic Interactions in Living Cells with a DNA Zipper Probe

Author: Yousef Bagheri, Ahsan Ausaf Ali, Puspam Keshri, James Chambers, Anne Gershenson, Mingxu You

Issue&Volume: 2021-11-12

Abstract: The cell membrane is a dynamic and heterogeneous structure composed of distinct sub-compartments.  Within these compartments, preferential interactions occur among various lipids and proteins.  Currently, it is still challenging to image these short-lived membrane complexes, especially in living cells.  In this work, we present a DNA-based probe, termed “DNA Zipper”, which allows the membrane order and pattern of transient interactions to be imaged in living cells using standard fluorescence microscopes.  By fine-tuning the length and binding affinity of DNA duplex, these probes can precisely extend the duration of membrane lipid interactions via dynamic DNA hybridization.  The correlation between membrane order and the activation of T-cell receptor signalling has also been studied.  These programmable DNA probes function after a brief cell incubation, which can be easily adapted to study lipid interactions and membrane order during different membrane signalling events.

DOI: 10.1002/anie.202112033

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202112033