英国爱丁堡大学Marc Vendrell团队报道了一种用于化疗诱导的癌细胞死亡的筛选和活体成像的二价可激活荧光探针。相关研究成果发表在2021年11月11日出版的《德国应用化学》。

凋亡细胞的检测和定量是癌症研究中的一个关键过程，尤其是在筛选抗癌药物和使用临床前模型进行机理研究的过程中。活体光学成像可实现活体细胞获得的高分辨率可视化；然而，很少有荧光探针能够在不受组织自体荧光干扰的情况下可靠地提供原位功能读出。

该文中，研究人员报告了实时检测癌细胞死亡的荧光探针ApoRedTracker的设计和优化。Apotracker Red的强荧光特性、高选择性和优异的稳定性使其成为一种可靠的光学报告器，用于体外细胞中抗癌药物作用的表征以及在乳腺癌小鼠模型中直接成像体内化疗诱导的凋亡。

附：英文原文

Title: A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy-induced Cancer Cell Death

Author: Nicole Barth, Lorena Mendive-Tapia, Ramon Subiros-Funosas, Ouldouz Ghashghaie, Rodolfo Lavilla, Laura Maiorino, Xue-Yan He, Ian Dransfield, Mikala Egeblad, Marc Vendrell

Issue&Volume: 2021-11-11

Abstract: The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high-resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. Here we report the design and optimisation of the fluorogenic probe Apotracker Red for real-time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.

DOI: 10.1002/anie.202113020

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202113020