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内在无序的缓步动物蛋白质在环境压力下自组装成纤维凝胶
作者:小柯机器人 发布时间:2021/11/12 13:46:58

法国IBSMartin Blackledge团队报道了内在无序的缓步动物蛋白质在环境压力下自组装成纤维凝胶。相关研究成果于2021年11月9日发表在《德国应用化学》。

缓步类动物以其在极端温度和干燥等极端压力条件下的生存能力而著称。赋予这种不寻常的抵抗物理压力的分子机制仍然未知。近年来,缓步动物特有的内在无序蛋白质在缓步动物脱水过程中起着重要作用。

该文中,研究人员描述了典型催眠中CAHS-8的构象和物理行为。核磁共振波谱显示,该蛋白质包含一个扩展的中心螺旋结构域,两侧是无序的末端。浓缩后,蛋白质以强烈的温度依赖性方式依次形成低聚物、长纤维和最终由纤维构成的凝胶。螺旋结构域形成纤维结构的核心,无序的末端在凝胶中保持高度动态。可溶性蛋白质可以包裹在凝胶的空腔中,保持其功能形态。可逆形成纤维凝胶的能力可能与增强这些蛋白质的保护性能有关。

附:英文原文

Title: Intrinsically Disordered Tardigrade Proteins Self-Assemble into Fibrous Gels in Response to Environmental Stress

Author: Anas Malki, Jean-Marie Teulon, Aldo Camacho Zarco, Shu-wen W. Chen, Wiktor Adamski, Damien Maurin, Nicola Salvi, Jean-Luc Pellequer, Martin Blackledge

Issue&Volume: 2021-11-09

Abstract: Tardigrades are remarkable for their ability to survive drastic stress conditions as diverse as extreme temperature and desiccation. The molecular mechanisms that confer this unusual resistance to physical stress remain unknown. Recently, tardigrade-unique intrinsically disordered proteins have been shown to play an essential role in tardigrade anhydrobiosis. Here, we characterize the conformational and physical behaviour of CAHS-8 from  Hypsibius exemplaris.  NMR spectroscopy reveals that the protein comprises an extended central helical domain, flanked by disordered termini. Upon concentration, the protein is shown to successively form oligomers, long fibres and finally gels constituted of fibres, in a strongly temperature-dependent manner. The helical domain forms the core of the fibrillar structure, with the disordered termini remaining highly dynamic within the gel. Soluble proteins can be encapsulated within cavities in the gel, maintaining their functional form. The ability to reversibly form fibrous gels may be associated with the enhanced protective properties of these proteins.

DOI: 10.1002/anie.202109961

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202109961

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx