瑞士联邦理工学院Elena Goun研究团队取得一项新突破，他们实现了对体内葡萄糖摄取行为的生物荧光成像与定量研究。 2019年6月，国际知名学术期刊《Nature Methods》发表了这一成果。

该团队研发了一种监测葡萄糖摄取的生物荧光探针，其可对体内外的葡萄糖摄取行为进行实时、无创的纵向成像。此外，研究表明该方法的灵敏度与常用的18F-FDG（氟18标记的葡萄糖）PET示踪剂相当，并且该探针可用来鉴定新型的葡萄糖受体抑制剂。这一研究成果在代谢和药物开发领域有一定的应用前景。

据报道，葡萄糖是大多数生物体的主要能量来源，其异常摄取与许多病理状态有关。然而，由于缺乏强有力的工具，我们对与疾病相关葡萄糖摄入流的改变知之甚少。到目前为止，正电子发射断层成像（PET）仍然是测量葡萄糖摄取的金标准，并且没有光学成像手段可以实现对体内葡萄糖摄取行为的无创纵向成像。

附：英文原文

Title: Bioluminescent-based imaging and quantification of glucose uptake in vivo

Author: Tamara Maric, Georgy Mikhaylov, Pavlo Khodakivskyi, Arkadiy Bazhin, Riccardo Sinisi, Nicolas Bonhoure, Aleksey Yevtodiyenko, Anthony Jones, Vishaka Muhunthan, Gihad Abdelhady, David Shackelford, Elena Goun

Issue&Volume: Volume 16 Issue 6, June 2019

Abstract: Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.

DOI: 10.1038/s41592-019-0421-z

Source:https://www.nature.com/articles/s41592-019-0421-z