意大利圣拉斐尔基因治疗研究所Andrea Ditadi等研究人员合作发现，CD32可捕获人类胚胎发育过程中确立的造血内皮细胞。该项研究成果于2024年4月9日在线发表在《自然—细胞生物学》杂志上。

研究人员对发育28到32天的人类胚胎进行了转录组分析，观察到Fc受体CD32（FCGR2B）的表达在包含造血内皮细胞（HEC）的内皮细胞群中高度富集。利用人类胚胎和人类多能干细胞衍生的内皮细胞进行的功能分析显示，CD32⁺内皮细胞中蕴藏着强大的多谱系造血潜能，并显示90%的CD32⁺内皮细胞是真正的HEC。

值得注意的是，这些分析表明，HEC经历了不同的状态，最终达到FCGR2B表达，此时细胞不可逆转地致力于造血命运。这些发现为从人类胚胎和人类多能干细胞培养物中分离HEC提供了一种精确的方法，从而可以在体外高效地生成造血细胞。

研究人员表示，在胚胎发育过程中，血细胞从特化的内皮细胞中产生，这些细胞被命名为HEC。由于HEC很少见，而且只能在早期发育的胚胎中短暂发现，因此仍很难将其与内皮细胞区分开来。

附：英文原文

Title: CD32 captures committed haemogenic endothelial cells during human embryonic development

Author: Scarf, Rebecca, Randolph, Lauren N., Abou Alezz, Monah, El Khoury, Mahassen, Gersch, Amlie, Li, Zhong-Yin, Luff, Stephanie A., Tavosanis, Andrea, Ferrari Ramondo, Giulia, Valsoni, Sara, Cascione, Sara, Didelon, Emma, Passerini, Laura, Amodio, Giada, Brandas, Chiara, Villa, Anna, Gregori, Silvia, Merelli, Ivan, Freund, Jean-Nol, Sturgeon, Christopher M., Tavian, Manuela, Ditadi, Andrea

Issue&Volume: 2024-04-09

Abstract: During embryonic development, blood cells emerge from specialized endothelial cells, named haemogenic endothelial cells (HECs). As HECs are rare and only transiently found in early developing embryos, it remains difficult to distinguish them from endothelial cells. Here we performed transcriptomic analysis of 28- to 32-day human embryos and observed that the expression of Fc receptor CD32 (FCGR2B) is highly enriched in the endothelial cell population that contains HECs. Functional analyses using human embryonic and human pluripotent stem cell-derived endothelial cells revealed that robust multilineage haematopoietic potential is harboured within CD32+ endothelial cells and showed that 90% of CD32+ endothelial cells are bona fide HECs. Remarkably, these analyses indicated that HECs progress through different states, culminating in FCGR2B expression, at which point cells are irreversibly committed to a haematopoietic fate. These findings provide a precise method for isolating HECs from human embryos and human pluripotent stem cell cultures, thus allowing the efficient generation of haematopoietic cells in vitro.

DOI: 10.1038/s41556-024-01403-0

Source: https://www.nature.com/articles/s41556-024-01403-0