浙江大学张进等研究人员合作发现，低投入脂质组学揭示哺乳动物早期胚胎发育过程中的脂质代谢重塑。2024年2月1日，《自然—细胞生物学》杂志在线发表了这项成果。

研究人员全面揭示了小鼠和人类早期胚胎发育过程中的脂质动态变化。不同发育阶段的脂质特征各不相同，尤其是磷脂类。研究人员强调，当胚胎发育到囊胚阶段时，磷脂的高度不饱和是一个保守的特征。此外，研究人员还发现，脂质去饱和酶（如SCD1）是体外囊胚发育和囊胚植入所必需的。其机制之一是通过调节不饱和脂肪酸介导的质膜和顶端蛋白的流动性，以及在八细胞胚胎发育到囊胚期间建立顶端-基底极性。

总之，这项研究为哺乳动物植入前胚胎发育过程中内源性脂质体的重塑提供了宝贵的资源，并为脂质不饱和对胚胎发生和植入的调控提供了机理上的启示。

据了解，脂质是能量储存、膜结构和细胞信号传递不可或缺的物质。然而，哺乳动物早期胚胎发育过程中各类内源性脂质的动态变化尚未得到系统研究。

附：英文原文

Title: Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development

Author: Zhang, Ling, Zhao, Jing, Lam, Sin Man, Chen, Lang, Gao, Yingzhuo, Wang, Wenjie, Xu, Yuyan, Tan, Tianyu, Yu, Hua, Zhang, Min, Liao, Xufeng, Wu, Mengchen, Zhang, Tianyun, Huang, Jie, Li, Bowen, Zhou, Quan D., Shen, Ning, Lee, Hyeon Jeong, Ye, Cunqi, Li, Da, Shui, Guanghou, Zhang, Jin

Issue&Volume: 2024-02-01

Abstract: Lipids are indispensable for energy storage, membrane structure and cell signalling. However, dynamic changes in various categories of endogenous lipids in mammalian early embryonic development have not been systematically characterized. Here we comprehensively investigated the dynamic lipid landscape during mouse and human early embryo development. Lipid signatures of different developmental stages are distinct, particularly for the phospholipid classes. We highlight that the high degree of phospholipid unsaturation is a conserved feature as embryos develop to the blastocyst stage. Moreover, we show that lipid desaturases such as SCD1 are required for in vitro blastocyst development and blastocyst implantation. One of the mechanisms is through the regulation of unsaturated fatty-acid-mediated fluidity of the plasma membrane and apical proteins and the establishment of apical–basal polarity during development of the eight-cell embryo to the blastocyst. Overall, our study provides an invaluable resource about the remodelling of the endogenous lipidome in mammalian preimplantation embryo development and mechanistic insights into the regulation of embryogenesis and implantation by lipid unsaturation.

DOI: 10.1038/s41556-023-01341-3

Source: https://www.nature.com/articles/s41556-023-01341-3