俄罗斯国家研究中心Fedor V. Subach和西湖大学Kiryl D. Piatkevich研究团队合作取得一项新突破。他们发现了一种来自StayGold高亮且稳定的单体绿色荧光蛋白。2024年2月26日，国际学术期刊《自然-方法学》发表了这一成果。

研究人员发现了一种名为mBaoJin的StayGold单体变体并解析了其晶体结构，这种变体既保留了其前体的有益特性，又可用作结构蛋白和膜蛋白标签。研究人员将mBaoJin与通用的绿色荧光蛋白，以及其他新近推出的StayGold单体和假单体衍生物进行了系统比对，结果表明mBaoJin是一种高亮、光稳定的荧光蛋白，具有快速成熟和高pH值/化学稳定的特点。研究进一步展示了mBaoJin在模式生物（包括秀丽隐杆线虫和小鼠）神经元标记中的适用性。

研究人员表示，绿色荧光蛋白StayGold的高亮度和光稳定性使其成为一种具有潜力的探针，可用于长期活细胞成像。然而，它二聚体的性质使其无法用作某些蛋白质的荧光标签。

附：英文原文

Title: Bright and stable monomeric green fluorescent protein derived from StayGold

Author: Zhang, Hanbin, Lesnov, Gleb D., Subach, Oksana M., Zhang, Wenhao, Kuzmicheva, Tatyana P., Vlaskina, Anna V., Samygina, Valeriya R., Chen, Liangyi, Ye, Xianxin, Nikolaeva, Alena Yu., Gabdulkhakov, Azat, Papadaki, Stavrini, Qin, Wenming, Borshchevskiy, Valentin, Perfilov, Maxim M., Gavrikov, Alexey S., Drobizhev, Mikhail, Mishin, Alexander S., Piatkevich, Kiryl D., Subach, Fedor V.

Issue&Volume: 2024-02-26

Abstract: The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditiselegans and mice.

DOI: 10.1038/s41592-024-02203-y

Source: https://www.nature.com/articles/s41592-024-02203-y