美国基因泰克公司Frederic J. de Sauvage研究组在研究中取得进展。他们研制发现肾上腺素能神经通过3型先天淋巴细胞（ILC3s）分泌的IL-22信号传导调节肠道再生。2023年8月18日，国际学术期刊《细胞-干细胞》发表了这一成果。

研究人员探究了肠道支配肾上腺素能神经对照射（IR）诱导损伤后上皮细胞修复的影响。研究人员观察到，IR后小肠中的肾上腺素能神经密度增加，而化学肾上腺素能去神经支配损害了上皮再生。单细胞RNA测序结果显示，去神经支配动物在IR后IL-22信号传导减少。药理和遗传学工具相结合，研究人员证明β-肾上腺素能受体信号传导促进IR后ILC3s产生IL-22，进而促进上皮再生。

这些结果揭示了对肠道再生非常重要的肾上腺素能-ILC3通路。

据介绍，肠上皮细胞具有较高的内在周转率，上皮的准确更新取决于微环境。肠道由密集的周围神经网络支配，该网络控制肠道生理学的各个方面。然而，神经元在调节上皮细胞再生中的作用仍然未知。

附：英文原文

Title: Adrenergic nerves regulate intestinal regeneration through IL-22 signaling from type 3 innate lymphoid cells

Author: Putianqi Wang, Noelyn Kljavin, Thi Thu Thao Nguyen, Elaine E. Storm, Bryan Marsh, Jian Jiang, William Lin, Hari Menon, Robert Piskol, Frederic J. de Sauvage

Issue&Volume: 2023-08-18

Abstract: The intestinal epithelium has high intrinsic turnover rate, and the precise renewalof the epithelium is dependent on the microenvironment. The intestine is innervatedby a dense network of peripheral nerves that controls various aspects of intestinalphysiology. However, the role of neurons in regulating epithelial cell regenerationremains largely unknown. Here, we investigated the effects of gut-innervating adrenergicnerves on epithelial cell repair following irradiation (IR)-induced injury. We observedthat adrenergic nerve density in the small intestine increased post IR, while chemicaladrenergic denervation impaired epithelial regeneration. Single-cell RNA sequencingexperiments revealed a decrease in IL-22 signaling post IR in denervated animals.Combining pharmacologic and genetic tools, we demonstrate that β-adrenergic receptorsignaling drives IL-22 production from type 3 innate lymphoid cells (ILC3s) post IR,which in turn promotes epithelial regeneration. These results define an adrenergic-ILC3axis important for intestinal regeneration.

DOI: 10.1016/j.stem.2023.07.013

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00258-8