当前位置:科学网首页 > 小柯机器人 >详情
植原体通过不依赖泛素的蛋白质降解来调节宿主的发育
作者:小柯机器人 发布时间:2021/9/24 13:28:42

英国诺里奇科研公园Saskia A. Hogenhout研究小组发现,植原体通过不依赖泛素的蛋白质降解来调节宿主的发育。这一研究成果于2021年9月17日在线发表在国际学术期刊《细胞》上。

研究人员展示了来自昆虫传播的植物病原体SAP05蛋白效应子如何控制几个植物的发育过程。这些效应子同时延长了宿主的寿命,并诱导叶片和不育芽(由植物原生质体和载体定植的器官)的丛枝式增殖。SAP05通过介导SPL和GATA发育调节因子的同时降解而发挥作用,这个过程依赖于劫持植物泛素受体RPN10而不依赖于底物泛素化。

RPN10在真核生物中是高度保守的,但SAP05不与昆虫载体RPN10结合。植物RPN10内的两个氨基酸替换产生了一个对SAP05活动有抵抗力的功能变体。因此,一个效应蛋白使专职寄生植原体在其宿主中诱发大量的发育表型。

据介绍,某些专职植原体在它们的宿主身上诱发复杂和实质性的表型变化,有利于它们向其他营养级的传播。然而,这些变化的机制在很大程度上仍然是未知的。

附:英文原文

Title: Parasitic modulation of host development by ubiquitin-independent protein degradation

Author: Weijie Huang, Allyson M. MacLean, Akiko Sugio, Abbas Maqbool, Marco Busscher, Shu-Ting Cho, Sophien Kamoun, Chih-Horng Kuo, Richard G.H. Immink, Saskia A. Hogenhout

Issue&Volume: 2021-09-17

Abstract: Certain obligate parasites induce complex and substantial phenotypic changes in their hosts in ways that favor their transmission to other trophic levels. However, the mechanisms underlying these changes remain largely unknown. Here we demonstrate how SAP05 protein effectors from insect-vectored plant pathogenic phytoplasmas take control of several plant developmental processes. These effectors simultaneously prolong the host lifespan and induce witches’ broom-like proliferations of leaf and sterile shoots, organs colonized by phytoplasmas and vectors. SAP05 acts by mediating the concurrent degradation of SPL and GATA developmental regulators via a process that relies on hijacking the plant ubiquitin receptor RPN10 independent of substrate ubiquitination. RPN10 is highly conserved among eukaryotes, but SAP05 does not bind insect vector RPN10. A two-amino-acid substitution within plant RPN10 generates a functional variant that is resistant to SAP05 activities. Therefore, one effector protein enables obligate parasitic phytoplasmas to induce a plethora of developmental phenotypes in their hosts.

DOI: 10.1016/j.cell.2021.08.029

Source: https://www.cell.com/cell/fulltext/S0092-8674(21)01012-6

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/