华中师范大学李海兵团队受核孔复合体（NPC）的启发，开发了高通量蛋白质转运通道的构建策略。相关研究成果于2021年9月16日发表于国际顶尖学术期刊《德国应用化学》。

受核孔复合体（NPC）的启发，研究人员通过选择性地将柱[5]芳烃主客体系统引入人工凹面（小时玻璃）形纳米通道的一侧，建立了仿生高通量蛋白质输送系统。该系统以每分钟660溶菌酶的传输通量提供高效的高通量蛋白质传输，传输速率显著高于未修饰的纳米通道和传统的双侧对称修饰的纳米通道。

鉴于该有前景的结果，利用人工纳米通道改善蛋白质运输不仅为生物学研究和更好地理解蛋白质在生命系统中的运输行为提供了一种新的潜在化学模型，而且还提供了一种高通量蛋白质运输装置，在不久的将来，它可能在蛋白质药物释放系统、蛋白质分离系统和微流体的设计中获得应用。

附：英文原文

Title: Construction of A High-flux Protein Transport Channel Inspired by the Nuclear Pore Complex

Author: Lei Yang, Ming Cheng, Jiaxin Quan, Siyun Zhang, Lu Liu, Robert P. Johnson, Fan Zhang, Haibing Li

Issue&Volume: 2021-09-16

Abstract: Inspired by the nuclear pore complex (NPC), herein we have established a biomimetic high-flux protein delivery system via the selective introduction of pillar[5]arene-based host-guest system into one side of an artificial concave (hour-glass) shaped nanochannels. With a transport flux of 660 lysozymes per minute, the system provides efficient high-flux protein transport at a rate which is significantly higher than that of an unmodified nanochannel and conventional bilateral symmetrical modified nanochannels. In view of these promising results, the use of artificial nanochannels to improve protein transport not only provides a new potential chemical model for biological research and better understanding of protein transport behavior in the living systems, but also provides a high-flux protein transporter device, which may have applications in the design of protein drug release systems, protein separation systems and microfluidics in the near future.

DOI: 10.1002/anie.202110273

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202110273