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研究绘制出人类肠道细胞跨越空间和时间的分布图谱
作者:小柯机器人 发布时间:2021/9/12 12:52:35

英国威康桑格研究所Sarah A. Teichmann、Kylie R. James等研究人员合作绘制出人类肠道细胞跨越空间和时间的分布图谱。该项研究成果发表在2021年9月8日出版的《自然》杂志上。

为了全面绘制细胞谱系,研究人员使用单细胞RNA测序和抗原受体分析,对来自发育中的5个解剖区域以及健康的儿童和成人肠道的11个不同解剖区域的近50万个细胞进行了分析。这揭示了整个人类肠道存在转录不同的BEST4上皮细胞。此外,研究人员发现IgG感应是肠道簇状细胞的一种功能。研究人员描述了发育中的肠道神经系统的神经细胞群,并预测了与赫希斯庞氏病相关基因的细胞类型特异表达。

最后,利用系统方法,研究人员确定了在人类早期发育中驱动次级淋巴组织形成的关键细胞参与者。结果表明,这些程序在炎症性肠病中被使用,从而在炎症部位招募和维持免疫细胞。这个肠道细胞的图谱将为发育、平衡和疾病中的细胞程序提供新的见解。

据介绍,人类肠道的细胞分布在整个生命过程中是动态的,在子宫内发育,并随着功能需求和环境暴露而改变。

附:英文原文

Title: Cells of the human intestinal tract mapped across space and time

Author: Elmentaite, Rasa, Kumasaka, Natsuhiko, Roberts, Kenny, Fleming, Aaron, Dann, Emma, King, Hamish W., Kleshchevnikov, Vitalii, Dabrowska, Monika, Pritchard, Sophie, Bolt, Liam, Vieira, Sara F., Mamanova, Lira, Huang, Ni, Perrone, Francesca, Goh KaiEn, Issac, Lisgo, Steven N., Katan, Matilda, Leonard, Steven, Oliver, Thomas R. W., Hook, C. Elizabeth, Nayak, Komal, Campos, Lia S., Domnguez Conde, Cecilia, Stephenson, Emily, Engelbert, Justin, Botting, Rachel A., Polanski, Krzysztof, van Dongen, Stijn, Patel, Minal, Morgan, Michael D., Marioni, John C., Bayraktar, Omer Ali, Meyer, Kerstin B., He, Xiaoling, Barker, Roger A., Uhlig, Holm H., Mahbubani, Krishnaa T., Saeb-Parsy, Kourosh, Zilbauer, Matthias, Clatworthy, Menna R., Haniffa, Muzlifah, James, Kylie R., Teichmann, Sarah A.

Issue&Volume: 2021-09-08

Abstract: The cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung’s disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease.

DOI: 10.1038/s41586-021-03852-1

Source: https://www.nature.com/articles/s41586-021-03852-1

期刊信息

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html