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科学家开发出生物动态实时多角度投影成像的新技术
作者:小柯机器人 发布时间:2021/6/30 23:43:26

美国德克萨斯大学西南医学中心Reto Fiolka研究小组开发出生物动态实时多角度投影成像的新技术。相关论文于2021年6月28日在线发表在《自然—方法学》杂志上。

研究人员推出了一种经济高效且易于实施的扫描单元,可将任何具有光学切片功能的相机显微镜转换为多角度投影成像系统。投影成像减少了数据开销并将成像速度提高了100倍以上,同时还允许用户随时从多个角度轻松查看感兴趣的生物现象。通过仅从两个角度快速询问样品,这个方法还可以实现实时立体成像和三维粒子定位。

研究人员展示了使用旋转圆盘共聚焦、晶格光片、多向照明光片和斜平面显微镜对从单细胞中的细胞器到斑马鱼胚胎的血管系统的标本进行投影成像。

此外,研究人员利用这个投影方法以高达119Hz的速率快速成像了癌细胞形态动力学和培养神经元中的钙信号,并同时对跳动的胚胎斑马鱼心脏的正交视图进行了成像。

附:英文原文

Title: Real-time multi-angle projection imaging of biological dynamics

Author: Bo-Jui Chang, James D. Manton, Etai Sapoznik, Theresa Pohlkamp, Tamara S. Terrones, Erik S. Welf, Vasanth S. Murali, Philippe Roudot, Kayley Hake, Lachlan Whitehead, Andrew G. York, Kevin M. Dean, Reto Fiolka

Issue&Volume: 2021-06-28

Abstract: We introduce a cost-effective and easily implementable scan unit that converts any camera-based microscope with optical sectioning capability into a multi-angle projection imaging system. Projection imaging reduces data overhead and accelerates imaging by a factor of >100, while also allowing users to readily view biological phenomena of interest from multiple perspectives on the fly. By rapidly interrogating the sample from just two perspectives, our method also enables real-time stereoscopic imaging and three-dimensional particle localization. We demonstrate projection imaging with spinning disk confocal, lattice light-sheet, multidirectional illumination light-sheet and oblique plane microscopes on specimens that range from organelles in single cells to the vasculature of a zebrafish embryo. Furthermore, we leverage our projection method to rapidly image cancer cell morphodynamics and calcium signaling in cultured neurons at rates up to 119Hz as well as to simultaneously image orthogonal views of a beating embryonic zebrafish heart.

DOI: 10.1038/s41592-021-01175-7

Source: https://www.nature.com/articles/s41592-021-01175-7

期刊信息

Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex