美国丹娜-法伯癌症研究所X. Shirley Liu研究组开发出从不同类型RNA-seq数据中进行免疫库重建的新方法。该研究于2021年5月13日在线发表于国际一流学术期刊《自然—方法学》。

研究人员报道了TRUST4开源算法，可用于从RNA序列（RNA-seq）数据重建αβ/γδT细胞和B细胞中的免疫受体库。与竞争方法相比，TRUST4支持FASTQ和BAM格式，并且在组装更长（甚至全长）受体库时更快速，更灵敏。

TRUST4还可以在不进行V(D)J富集的情况下从单细胞RNA-seq（scRNA-seq）数据调用库序列，并且与SMART-seq和5'10xGenomics平台兼容。

附：英文原文

Title: TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data

Author: Li Song, David Cohen, Zhangyi Ouyang, Yang Cao, Xihao Hu, X. Shirley Liu

Issue&Volume: 2021-05-13

Abstract: We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ Tcells and Bcells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5′10xGenomics platforms.

DOI: 10.1038/s41592-021-01142-2

Source: https://www.nature.com/articles/s41592-021-01142-2