澳大利亚昆士兰大学Chun-Xia Zhao研究小组的一项最新研究，探讨了猝灭-去猝灭转变在染料标记纳米颗粒的定量细胞摄取和生物分布中的意义。该研究于2021年4月1日发表于国际一流学术期刊《德国应用化学》。

在该文中，研究人员比较了两种类型的染料标记纳米颗粒（NPs），并展示了它们不同的特性。具有固态染料分子的NPs会遭受染料淬灭，因此即便在同样数量的NPs下，生物环境中的染料释放或NP降解会导致荧光强度增强数倍。相反，染料分子处于可溶状态的NPs没有猝灭作用。

为了使对照研究更加标准化，研究人员提出了两种可能的解决方案：降低染料负载量，或用培养基分析来定量NPs的细胞摄取。这项工作为生物纳米研究中选择有效的定量方法提供了有价值的见解。

据悉，开展细胞摄取和生物分布研究的一般策略是用荧光染料标记的纳米颗粒。然而，由于不受控制的染料猝灭和去猝灭，对不同染料负载NPs的比较研究仍然很困难。

附：英文原文

Title: Implications of Quenching‐to‐Dequenching Switch in Quantitative Cell Uptake and Biodistribution of Dye‐Labeled Nanoparticles

Author: Guangze Yang, Yun Liu, Yue Hui, . Tengjisi, Dong Chen, David A. Weitz, Chun-Xia Zhao

Issue&Volume: 2021-04-01

Abstract: A general strategy to carry out cell uptake and biodistribution studies is to label nanoparticles (NPs) with a fluorescent dye. However, the comparative study of different dye‐loaded NPs remains difficult due to uncontrolled dye quenching and de‐quenching. Here we compared two types of dye‐labeled NPs and demonstrated their distinct properties. NPs with dye molecules at a solid state suffer from dye quenching, so the dye release and/or NP degradation in biological environments leads to a several‐fold increase of fluorescence intensity despite the same amount of NPs, due to the state switch from quenching to de‐quenching. In contrast, NPs with dye molecules at a soluble state exhibit no quenching effect. To standardize the comparative study, we propose two possible solutions: using lower dye loading, or using medium analysis for quantifying cell uptake of NPs. This work provides valuable insights into selecting valid quantification methods for bio‐nano studies.

DOI: 10.1002/anie.202101730

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202101730