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初始多能干细胞可在体外重现人类滋养细胞的发育
作者:小柯机器人 发布时间:2021/4/11 18:07:38

2021年4月7日,《细胞—干细胞》杂志在线发表了日本科学家的一项最新研究成果。来自京都大学的Yasuhiro Takashima课题组通过初始多能干细胞在体外重现人类滋养细胞的发育。

据研究人员介绍,滋养细胞是胚外细胞,对于维持妊娠至关重要。人体滋养细胞从桑椹胚中以滋养外胚层(TE)的形式出现,着床后可分化为构成胎盘的滋养细胞(CT)、合胞体滋养细胞(ST)和绒毛外滋养细胞(EVT)。

研究人员发现,初始但未活化的人多能干细胞(PSC)概括了滋养细胞的发育。初始PSC来源的TE和CT(nCT)重现了人类和猴子从TE到CT的过渡。nCT可自我更新为CT干细胞,并具有增殖绒毛CT和妊娠早期细胞中CT的特征。

值得注意的是,尽管活化的PSC分化为滋养细胞样细胞(BMP4、A83-01和PD173074 [BAP]处理的活化PSC [pBAP]),但pBAP与nCT和人胎盘来源的CT干细胞不同,其表现出与羊膜一致的特征。这些发现建立了人类滋养细胞发育的真实范例,并证明了初始人类PSC的宝贵价值。这个系统提供了一个平台,可用于研究滋养细胞发育和相关疾病的分子机制。 

附:英文原文

Title: Capturing human trophoblast development with naive pluripotent stem cells in vitro

Author: Shingo Io, Mio Kabata, Yoshiki Iemura, Katsunori Semi, Nobuhiro Morone, Atsutaka Minagawa, Bo Wang, Ikuhiro Okamoto, Tomonori Nakamura, Yoji Kojima, Chizuru Iwatani, Hideaki Tsuchiya, Belinda Kaswandy, Eiji Kondoh, Shin Kaneko, Knut Woltjen, Mitinori Saitou, Takuya Yamamoto, Masaki Mandai, Yasuhiro Takashima

Issue&Volume: 2021-04-07

Abstract: Trophoblasts are extraembryonic cells that are essential for maintaining pregnancy.Human trophoblasts arise from the morula as trophectoderm (TE), which, after implantation,differentiates into cytotrophoblasts (CTs), syncytiotrophoblasts (STs), and extravilloustrophoblasts (EVTs), composing the placenta. Here we show that nave, but not primed,human pluripotent stem cells (PSCs) recapitulate trophoblast development. Naive PSC-derivedTE and CTs (nCTs) recreated human and monkey TE-to-CT transition. nCTs self-renewedas CT stem cells and had the characteristics of proliferating villous CTs and CTsin the cell column of the first trimester. Notably, although primed PSCs differentiatedinto trophoblast-like cells (BMP4, A83-01, and PD173074 [BAP]-treated primed PSCs[pBAPs]), pBAPs were distinct from nCTs and human placenta-derived CT stem cells,exhibiting properties consistent with the amnion. Our findings establish an authenticparadigm for human trophoblast development, demonstrating the invaluable propertiesof naive human PSCs. Our system provides a platform to study the molecular mechanismsunderlying trophoblast development and related diseases.

DOI: 10.1016/j.stem.2021.03.013

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00119-3

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx