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DNA砖辅助离心法分离不同粒径的亚150 nm脂质体
作者:小柯机器人 发布时间:2021/3/31 15:43:17

2021年3月30日出版的《自然-化学》杂志报道了美国耶鲁大学医学院Chenxiang Lin团队和复旦大学生物医学研究所Hongzhou Gu团队的最新成果。他们的研究显示,DNA砖辅助离心实现不同大小的亚150 nm脂质体的分选。

课题组报道了一种通用的、可扩展的分类技术,使用胆固醇修饰的DNA“纳米砖”,通过其浮力密度来区分不同大小的脂质体。不论脂质体的来源和化学成分如何,这种方法将脂质体分离成6到8个平均直径为30 - 130nm的均质群体。研究人员表明,这些均匀、抗泄漏的脂质体可作为理想的底物,以前所未有的分辨率研究膜曲率如何影响外周(ATG3)和整体(SNARE)膜蛋白活性。

与传统方法相比,他们的分选技术代表了简化的过程,可实现卓越的脂质体大小均匀性,这有利于膜生物学研究和脂质体药物递送系统的开发。                                              

据介绍,在细胞中,无数的膜相互作用蛋白产生并维持曲率半径在50nm左右或以下的弯曲膜结构域。为了了解这种高度弯曲的膜如何调节特定的蛋白质功能,反之亦然,必须将具有精确定义属性的小型脂质体作为模型膜。

附:英文原文

Title: Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation

Author: Yang Yang, Zhenyong Wu, Laurie Wang, Kaifeng Zhou, Kai Xia, Qiancheng Xiong, Longfei Liu, Zhao Zhang, Edwin R. Chapman, Yong Xiong, Thomas J. Melia, Erdem Karatekin, Hongzhou Gu, Chenxiang Lin

Issue&Volume: 2021-03-30

Abstract: In cells, myriad membrane-interacting proteins generate and maintain curved membrane domains with radii of curvature around or below 50nm. To understand how such highly curved membranes modulate specific protein functions, and vice versa, it is imperative to use small liposomes with precisely defined attributes as model membranes. Here, we report a versatile and scalable sorting technique that uses cholesterol-modified DNA ‘nanobricks’ to differentiate hetero-sized liposomes by their buoyant densities. This method separates milligrams of liposomes, regardless of their origins and chemical compositions, into six to eight homogeneous populations with mean diameters of 30–130nm. We show that these uniform, leak-resistant liposomes serve as ideal substrates to study, with an unprecedented resolution, how membrane curvature influences peripheral (ATG3) and integral (SNARE) membrane protein activities. Compared with conventional methods, our sorting technique represents a streamlined process to achieve superior liposome size uniformity, which benefits research in membrane biology and the development of liposomal drug-delivery systems.

DOI: 10.1038/s41557-021-00667-5

Source: https://www.nature.com/articles/s41557-021-00667-5

期刊信息

Nature Chemistry:《自然—化学》,创刊于2009年。隶属于施普林格·自然出版集团,最新IF:21.687
官方网址:https://www.nature.com/nchem/
投稿链接:https://mts-nchem.nature.com/cgi-bin/main.plex