当前位置:科学网首页 > 小柯机器人 >详情
自降解多功能DNAzyme纳米海绵用于可编程药物递送和高效基因沉默
作者:小柯机器人 发布时间:2021/2/20 13:42:59

近日,武汉大学化学系教授Fuan Wang及其课题组提出了一种用于可编程药物递送和高效基因沉默的自降解多功能DNAzyme纳米海绵。 2021年2月18日出版的《德国应用化学》发表了这项成果。

该研究组设计了一种生物启发性的自异化DNA纳米胶囊,用于增强治疗性DNAzyme的肿瘤特异性级联激活。精美的DNAzyme在阶级式滚动环复制(RCR)纳米结构中被自我切除的DNAzyme暂时掩盖,它们在生理液体中保持不活跃状态。

通过多价肿瘤锚定核酸适配体链,RCR纳米胶囊特异性地积聚在癌细胞中,然后通过智能刺激响应级联DNAzyme激活而被有序地激活,以激发最终的DNAzyme介导的基因沉默。凭借可编程的RCR组装策略,其紧凑的DNAzyme纳米平台显示出开发多功能智能基因疗法和个性化纳米药物的巨大希望。

据悉,基于DNAzyme的基因疗法在有效治疗严重疾病方面具有广阔的前景,但受低效率递送和无条件激活的限制。

附:英文原文

Title: A self‐catabolic multifunctional DNAzyme nanosponge for programmable drug delivery and efficient gene silencing

Author: Jing Wang, Shanshan Yu, Qiong Wu, Xue Gong, Shizhen He, Jinhua Shang, Xiaoqing Liu, Fuan Wang

Issue&Volume: 2021-02-18

Abstract: DNAzyme‐based genetherapy holds immense prospects for effectively treating severe diseases, yet is constrained with inefficient delivery and unconditional activation. Herein, we designed a bioinspired self‐catabolic DNA nanocapsule for sustaining tumor‐specific cascade activation of therapeutic DNAzyme. The exquisite DNAzyme was temporarily masked by the self‐excising DNAzyme in the hierarchical rolling circle replication (RCR) nanostructures, thus stayed in an inactive state in physiological fluids. Through the multivalent tumor‐anchoring aptamer strands, the RCR nanocapsule was specifically accumulated in cancer cells and was sequentially activated for motivating the ultimate DNAzyme‐mediated gene silencing via the intelligent stimuli‐responsive cascade DNAzyme activation. By virtue of the programmable RCR assembly strategy, our compact DNAzyme nanoplatform shows great promise for developing versatile smart gene therapeutics and personalized nanomedicines.

DOI: 10.1002/anie.202101474

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202101474

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx