湖南大学邢航团队报道了利用融合球形核酸进行空间控制细胞膜工程的仿生方法。相关研究成果发表在2021年10月12日出版的《德国应用化学》。

利用功能性DNA构建细胞质膜对于研究和控制细胞行为具有重要意义。然而，由于缺乏合适的合成工具来设计细胞内侧，大多数研究限制了在细胞外膜上应用人工DNA相互作用，阻碍了在细胞生物学中的许多应用。

受自然细胞外囊泡细胞融合过程的启发，研究人员引入了融合球形核酸结构，通过脂质体融合转运（LiFT）在细胞内外表面实现稳健的DNA功能化，并实现包括为编程信号通路构建异型细胞组装和检测细胞内代谢物方面的应用。该方法可以以高效和空间控制的方式设计细胞膜，能够构建具有两种正交DNA功能的各向异性膜结构。

附：英文原文

Title: A Biomimetic Approach for Spatially-Controlled Cell Membrane Engineering Using Fusogenic Spherical Nucleic Acid

Author: Minjie Lin, Yuanyuan Chen, Sisi Zhao, Rui Tang, Zhou Nie, Hang Xing

Issue&Volume: 2021-10-12

Abstract: Engineering of cell plasma membrane using functional DNA is important for study and control of cellular behaviors. However, most efforts limit to apply artificial DNA interactions on external cell membrane due to the lack of suitable synthetic tools to engineer intracellular side, which impedes many applications in cell biology. Inspired by natural extracellular vesicle-cell fusion process, we introduce a fusogenic spherical nucleic acid construct to realize robust DNA functionalization on both external and internal cell surfaces via liposome fusion-based transport (LiFT), enabling applications including construction of heterotypic cell assembly for programmed signaling pathway and detection of intracellular metabolites. This approach can engineer cell membrane in a highly efficient and spatially-controlled manner, allowing for building anisotropic membrane structure with two orthogonal DNA functionalities.

DOI: 10.1002/anie.202111647

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202111647