美国纪念斯隆-凯特琳癌症中心Alexandros Pertsinidis团队开发出体积干涉测量格子光片成像技术。2021年10月11日，《自然—生物技术》杂志在线发表了这项成果。

研究人员提出了三维干涉晶格光片（3D-iLLS）成像，这是一种需要低激发光水平的技术，通过结合4Pi干涉法和选择性平面照明，提供高背景抑制和大幅提高体积分辨率。研究人员证明，3D-iLLS的轴向分辨率和单粒子定位精度分别为100纳米（FWHM）和<10纳米（1σ）。研究人员展示了3D-iLLS在一系列系统中的表现：单个信使RNA分子、细胞核中转录调节因子的纳米级组合、微管细胞骨架和线粒体细胞器。3D-iLLS增强的4D分辨率和增加的信噪比将促进对亚细胞水平的生物过程的分析。

据了解，具有高时空分辨率和高检测灵敏度的活细胞成像有助于研究细胞结构和功能的动态变化。然而，从活细胞中提取高分辨率的4D（三维空间加时间）信息仍然具有挑战性，因为目前的方法很慢，需要很高的峰值激发强度或受到高失焦背景的影响。

附：英文原文

Title: Volumetric interferometric lattice light-sheet imaging

Author: Cao, Bin, Coelho, Simao, Li, Jieru, Wang, Guanshi, Pertsinidis, Alexandros

Issue&Volume: 2021-10-11

Abstract: Live cell imaging with high spatiotemporal resolution and high detection sensitivity facilitates the study of the dynamics of cellular structure and function. However, extracting high-resolution 4D (3D space plus time) information from live cells remains challenging, because current methods are slow, require high peak excitation intensities or suffer from high out-of-focus background. Here we present 3D interferometric lattice light-sheet (3D-iLLS) imaging, a technique that requires low excitation light levels and provides high background suppression and substantially improved volumetric resolution by combining 4Pi interferometry with selective plane illumination. We demonstrate that 3D-iLLS has an axial resolution and single-particle localization precision of 100nm (FWHM) and <10nm (1σ), respectively. We illustrate the performance of 3D-iLLS in a range of systems: single messenger RNA molecules, nanoscale assemblies of transcription regulators in the nucleus, the microtubule cytoskeleton and mitochondria organelles. The enhanced 4D resolution and increased signal-to-noise ratio of 3D-iLLS will facilitate the analysis of biological processes at the sub-cellular level.

DOI: 10.1038/s41587-021-01042-y

Source: https://www.nature.com/articles/s41587-021-01042-y