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科学家绘制出单细胞分辨率下人类肠道发育的时空图谱
作者:小柯机器人 发布时间:2021/1/6 17:36:06

英国牛津大学Alison Simmons、HashemKoohy等研究人员合作绘制出单细胞分辨率下人类肠道发育的时空图谱。该研究于2021年1月5日在线发表于国际一流学术期刊《细胞》。

研究人员整合了单细胞RNA测序和空间转录组学来表征随时间变化的肠道形态发生。研究人员确定了101个细胞状态,包括上皮和间充质祖细胞群以及与关键形态发生学相关的程序。研究人员描述了隐窝-绒毛轴的形成,神经、血管、间充质的形态发生以及发育中的肠道免疫群体。

研究人员确定了发育为成纤维细胞和成肌纤维细胞亚型的分化层次,并描述了这些功能,包括血管微环境细胞的多种功能。研究人员揭示了Peyer斑和肠道相关淋巴组织(GALT)的起源,并描述了特定于位置的免疫程序。研究人员使用这一资源来呈现了形态发生素梯度的无偏倚分析,这一梯度能够指导细胞分化的连续波并定义与罕见发育性肠道疾病相关的细胞和位置。

研究人员汇编了一个公开可用的在线资源,即胎儿肠道发育的时空分析资源(STAR-FINDer),以希望促进今后的研究工作。

附:英文原文

Title: Spatiotemporal analysis of human intestinal development at single-cell resolution

Author: David Fawkner-Corbett, Agne Antanaviciute, Kaushal Parikh, Marta Jagielowicz, Ana Sousa Gerós, Tarun Gupta, Neil Ashley, Doran Khamis, Darren Fowler, Edward Morrissey, Chris Cunningham, Paul R.V. Johnson, Hashem Koohy, Alison Simmons

Issue&Volume: 2021-01-05

Abstract: Development of the human intestine is not well understood. Here, we link single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. We identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones. We describe principles of crypt-villus axis formation; neural, vascular, mesenchymal morphogenesis, and immune population of the developing gut. We identify the differentiation hierarchies of developing fibroblast and myofibroblast subtypes and describe diverse functions for these including as vascular niche cells. We pinpoint the origins of Peyer’s patches and gut-associated lymphoid tissue (GALT) and describe location-specific immune programs. We use our resource to present an unbiased analysis of morphogen gradients that direct sequential waves of cellular differentiation and define cells and locations linked to rare developmental intestinal disorders. We compile a publicly available online resource, spatio-temporal analysis resource of fetal intestinal development (STAR-FINDer), to facilitate further work.

DOI: 10.1016/j.cell.2020.12.016

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31686-X

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/