美国芝加哥大学Wenbin Lin研究团队在研究中取得进展。他们的研究开发出了纳米级金属有机层用于检测线粒体失调和化疗耐药性。 2021年1月15日，国际知名学术期刊《美国化学会杂志》发表了这一成果。

据介绍，线粒体失调通过改变内源分子浓度和跨膜离子流控制细胞的死亡和存活。

该课题组报告了一个三重发射纳米级金属有机层（nMOL），NA@Zr-BTB/F/R，用于感应线粒体失调。含有Zr₆二级结构单元（SBUs）和2,4,6-三（4-羧基苯基）苯胺（BTB-NH₂）配体的Zr-BTB nMOL经过合成后官能化，通过谷胱甘肽（GSH）-选择性（2E）-1-（2''-萘基）-3-（4-羧苯基）-2-丙烯-1-酮（NA）交换SBUs上的甲酸酯封端基团，以及共价缀合pH敏感荧光素（F）和不依赖GSH / pH的罗丹明B（R）到BTB-NH₂配体上，得到NA @ Zr-BTB / F / R。

细胞成像表明，NA @ Zr-BTB / F / R可作为比例传感器，通过传感GSH和pH，检测线粒体失调和化疗耐药性。

附：英文原文

Title: Nanoscale Metal–Organic Layers Detect Mitochondrial Dysregulation and Chemoresistance via Ratiometric Sensing of Glutathione and pH

Author: Xiang Ling, Deyan Gong, Wenjie Shi, Ziwan Xu, Wenbo Han, Guangxu Lan, Youyou Li, Wenwu Qin, Wenbin Lin

Issue&Volume: January 15, 2021

Abstract: Mitochondrial dysregulation controls cell death and survival by changing endogenous molecule concentrations and ion flows across the membrane. Here, we report the design of a triply emissive nanoscale metal–organic layer (nMOL), NA@Zr-BTB/F/R, for sensing mitochondrial dysregulation. Zr-BTB nMOL containing Zr₆ secondary building units (SBUs) and 2,4,6-tris(4-carboxyphenyl)aniline (BTB-NH₂) ligands was postsynthetically functionalized to afford NA@Zr-BTB/F/R by exchanging formate capping groups on the SBUs with glutathione(GSH)-selective (2E)-1-(2′-naphthyl)-3-(4-carboxyphenyl)-2-propen-1-one (NA) and covalent conjugation of pH-sensitive fluorescein (F) and GSH/pH-independent rhodamine-B (R) to the BTB-NH2 ligands. Cell imaging demonstrated NA@Zr-BTB/F/R as a ratiometric sensor for mitochondrial dysregulation and chemotherapy resistance via GSH and pH sensing.

DOI: 10.1021/jacs.0c11764

Source: https://pubs.acs.org/doi/10.1021/jacs.0c11764