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新研究揭示酶引导自组装实现单体和准分子转变
作者:小柯机器人 发布时间:2021/1/10 19:33:55

南方医科大学的Yanbin Cai团队和南开大学Zhimou Yang团队的一项最新研究,揭示了酶引导自组装实现单体和准分子转变,从而构建更有序的发光超分子组装,用于基于活性的生物成像。 相关论文于2021年1月07日发表于《德国应用化学》杂志。

课题组报道了酶指导的自组装(EISA)使香豆素染料(Cou)的在低分子浓度下单体-准分子跃迁成为可能,从而产生更有序的发光超分子组装(即纳米纤维),在体外和活体内有效记录碱性磷酸酶(ALP)的活性。Cou通过一个亲水ALP响应基团连接到自组装短肽上。通过ALP引发,EISA驱使在低肽浓度下纳米颗粒-纳米纤维转化,紧随着Cou单体-准分子转化。

结构-性能关系分析揭示了自组装模体是肽引发Cou单体-准分子转化的一个前提条件。发光pYD超分子纳米纤维(LSN-pYD)阐明了癌细胞分子间连接以及有效且快速地区分癌细胞(组织)和正常细胞(组织),对于癌症的早期检测就有前景。该工作拓展了EISA的功能并为超分子化学提供了新的应用。

据悉,如何构建在低分子浓度下具有高性能的基于准分子的发光分析工具仍然是一个挑战。

附:英文原文

Title: Enzyme Instructed Self‐assembly Enabled Monomer‐excimer Transition to Construct Higher Ordered Luminescent Supramolecular Assembly for Activity‐based Bioimaging

Author: Yuanzhi Zhong, Jie Zhan, Guanghui Xu, Yumiao Chen, Qin Qin, Xu Liao, Shaodan Ma, Zhimou Yang, Yanbin Cai

Issue&Volume: 07 January 2021

Abstract: How to construct high‐performing excimer‐based luminescent analytic tools at low molecular concentrations remains a challenge. Here, we reported that enzyme instructed self‐assembly (EISA) enabled the monomer‐excimer transition of a coumarin dye (Cou) at low molecular concentrations, and the resulting higher ordered luminescent supramolecular assemblies (i.e., nanofibers) efficiently recorded the spatiotemporal details of alkaline phosphatase (ALP) activity  in vitro  and  in vivo  .  Cou  was conjugated to short self‐assembly peptides with a hydrophilic ALP‐responsive group.  By ALP triggering, EISA actuated nanoparticles‐nanofibers transition at low peptides concentrations  followed by monomer‐excimer transition of  Cou  .  Analysis of structure‐property relationship revealed that the self‐assembly motif was a prerequisite for peptides to induce monomer‐excimer transition of  Cou  .  Luminescent supramolecular nanofibers of  pYD  (  LSN‐  pYD  ) illuminated the intercellular bridge of cancer cells and distinguished cancer cells (tissues) from normal cells (tissues) efficiently and rapidly, promising the potential use for early diagnosis of cancer.  This work extends the functions of EISA and provides a new application of supramolecular chemistry.

DOI: 10.1002/anie.202014278

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202014278

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx