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科学家解析SARS-CoV-2病毒的分子结构
作者：小柯机器人 发布时间：2020/9/15 15:16:05

清华大学李赛、浙江大学李兰娟等研究人员合作解析了SARS-CoV-2病毒的分子结构。2020年9月14日，《细胞》在线发表了这一成果。

通过使用冷冻电子断层扫描（cryo-ET）和子断层扫描图平均化（STA），研究人员报道了真实SARS-CoV-2病毒的分子组装。研究人员确定了融合前后构象中S蛋白的天然结构，其平均分辨率为8.7-11Å。通过质谱分析来自天然刺突的N-连接聚糖组成，研究人员发现天然聚糖与重组糖蛋白聚糖的总体加工状态高度相似。核糖核蛋白（RNP）及其更高级组装的天然构象也被揭示。
 
总体而言，这些特征非常详细地揭示了SARS-CoV-2病毒的结构，并阐明了该病毒如何在直径约80nm的管腔中堆积其约30kb长的单段RNA。
 
据悉，SARS-CoV-2是导致COVID-19大流行的包膜病毒。尽管在SARS-CoV-2蛋白的结构阐明方面取得了最新进展，但完整病毒的详细结构仍有待揭示。
 
附：英文原文

Title: Molecular architecture of the SARS-CoV-2 virus

Author: Hangping Yao, Yutong Song, Yong Chen, Nanping Wu, Jialu Xu, Chujie Sun, Jiaxing Zhang, Tianhao Weng, Zheyuan Zhang, Zhigang Wu, Linfang Cheng, Danrong Shi, Xiangyun Lu, Jianlin Lei, Max Crispin, Yigong Shi, Lanjuan Li, Sai Li

Issue&Volume: 2020-09-14

Abstract: SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryo-electron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in both pre- and postfusion conformations were determined to average resolutions of 8.7-11 . Compositions of the N-linked glycans from the native spikes were analyzed by mass-spectrometry, which revealed highly similar overall processing states of the native glycans to that of the recombinant glycoprotein glycans. The native conformation of the ribonucleoproteins (RNP) and its higher-order assemblies were revealed. Overall, these characterizations have revealed the architecture of the SARS-CoV-2 virus in exceptional detail, and shed lights on how the virus packs its ～30 kb long single-segmented RNA in the ～80 nm diameter lumen.

DOI: 10.1016/j.cell.2020.09.018

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31159-4

期刊信息
Cell：《细胞》，创刊于1974年。隶属于细胞出版社，最新IF：36.216
官方网址：https://www.cell.com/