日本名古屋大学Michitaka Notaguchi等研究人员发现，β-1,4-葡聚糖酶促进植物嫁接中的细胞粘附。这一研究成果于2020年8月7日发表在《科学》上。

研究人员发现，烟草能够移植各种被子植物。对移植物组合进行的转录组分析表明，分泌到细胞外区域的β-1,4-葡聚糖酶亚群有助于在移植物界面附近重建细胞壁。β-1,4-葡聚糖酶的过度表达促进了嫁接。使用烟草茎作为插穗，研究人员用其他植物科的根茎生产了番茄果实。这些发现表明，细胞-细胞粘附的过程是增强植物嫁接技术的潜在目标。

Title: Cell-cell adhesion in plant grafting is facilitated by β-1,4-glucanases

Author: Michitaka Notaguchi, Ken-ichi Kurotani, Yoshikatsu Sato, Ryo Tabata, Yaichi Kawakatsu, Koji Okayasu, Yu Sawai, Ryo Okada, Masashi Asahina, Yasunori Ichihashi, Ken Shirasu, Takamasa Suzuki, Masaki Niwa, Tetsuya Higashiyama

Issue&Volume: 2020/08/07

Abstract: Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue is attached to another through cell-cell adhesion. However, graft compatibility limits combinations to closely related species, and the mechanism is poorly understood. We found that Nicotiana is capable of graft adhesion with a diverse range of angiosperms. Comparative transcriptomic analyses on graft combinations indicated that a subclade of β-1,4-glucanases secreted into the extracellular region facilitates cell wall reconstruction near the graft interface. Grafting was promoted by overexpression of the β-1,4-glucanase. Using Nicotiana stem as an interscion, we produced tomato fruits on rootstocks from other plant families. These findings demonstrate that the process of cell-cell adhesion is a potential target to enhance plant grafting techniques.

DOI: 10.1126/science.abc3710

Source: https://science.sciencemag.org/content/369/6504/698