丹麦奥尔胡斯大学Simona Radutoiu和Kasper R. Andersen研究组取得最新进展。他们发现植物赖氨酸基序（LysM）受体中的配体识别基序是受体特异性的主要决定因素。相关论文于2020年8月7日发表于《科学》杂志上。

他们调查了莲花的几丁质和结瘤（Nod）因子受体如何启动免疫或根瘤共生的差异信号。这些受体的LysM1结构域中的两个基序决定了配体的特异性识别，并区分了它们在植物中的功能。 这些基序定义了配体结合位点，并构成了同源Nod因子受体中结构上最不相同的区域。相邻的基序可调节Nod因子识别的特异性，并确定豆类中相容性根瘤菌共生体的选择。

他们还确定了如何改变LysM受体的结合特异性，以促进Nod因子的识别和几丁质受体的信号传导，从而将工程性根瘤菌共生技术转化为非豆类的前景。

据介绍，植物进化出LysM受体以识别和解析微生物诱发因子，并驱动细胞内信号传导来限制或促进微生物定植。

附：英文原文

Title: Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Author: Zoltan Bozsoki, Kira Gysel, Simon B. Hansen, Damiano Lironi, Christina Krnauer, Feng Feng, Noor de Jong, Maria Vinther, Manoj Kamble, Mikkel B. Thygesen, Ebbe Engholm, Christian Kofoed, Sébastien Fort, John T. Sullivan, Clive W. Ronson, Knud J. Jensen, Mickal Blaise, Giles Oldroyd, Jens Stougaard, Kasper R. Andersen, Simona Radutoiu

Issue&Volume: 2020/08/07

Abstract: Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.

DOI: 10.1126/science.abb3377

Source: https://science.sciencemag.org/content/369/6504/663