英国牛津大学的Alison Simmons、Hashem Koohy等研究人员合作绘制出溃疡性结肠炎中结肠CD8+T细胞的单细胞图谱。相关论文于2020年8月3日在线发表于国际学术期刊《自然—医学》。
Title: Single-cell atlas of colonic CD8 + T cells in ulcerative colitis
Author: Daniele Corridoni, Agne Antanaviciute, Tarun Gupta, David Fawkner-Corbett, Anna Aulicino, Marta Jagielowicz, Kaushal Parikh, Emmanouela Repapi, Steve Taylor, Dai Ishikawa, Ryo Hatano, Taketo Yamada, Wei Xin, Hubert Slawinski, Rory Bowden, Giorgio Napolitani, Oliver Brain, Chikao Morimoto, Hashem Koohy, Alison Simmons
Abstract: Colonic antigen-experienced lymphocytes such as tissue-resident memory CD8+ Tcells can respond rapidly to repeated antigen exposure. However, their cellular phenotypes and the mechanisms by which they drive immune regulation and inflammation remain unclear. Here we compiled an unbiased atlas of human colonic CD8+ Tcells in health and ulcerative colitis (UC) using single-cell transcriptomics with T-cell receptor repertoire analysis and mass cytometry. We reveal extensive heterogeneity in CD8+ T-cell composition, including expanded effector and post-effector terminally differentiated CD8+ Tcells. While UC-associated CD8+ effector Tcells can trigger tissue destruction and produce tumor necrosis factor (TNF)-α, post-effector cells acquire innate signatures to adopt regulatory functions that may mitigate excessive inflammation. Thus, we identify colonic CD8+ T-cell phenotypes in health and UC, define their clonal relationships and characterize terminally differentiated dysfunctional UC CD8+ Tcells expressing IL-26, which attenuate acute colitis in a humanized IL-26 transgenic mouse model.