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中国科学家发现新的前列腺祖细胞类型
作者:小柯机器人 发布时间:2020/8/19 16:52:08

近日,中国科学院上海生化与细胞研究所高栋等研究人员合作,利用单细胞转录组学在远端前列腺内陷尖端中发现不同的腔祖细胞类型。这一研究成果于2020年8月17日在线发表在《自然—遗传学》上。

研究人员表征了来自小鼠前列腺的35129个细胞,并鉴定了以Tacstd2、Ck4和Psca表达为标志的独特的腔细胞类型(称为C型腔细胞,Luminal-C)。位于远端前列腺内陷尖端的Luminal-C细胞(称为Dist-Luminal-C)表现出更大的体外类器官形成能力和体内前列腺上皮导管再生能力。Luminal-C细胞的谱系追踪表明,Dist-Luminal-C细胞通过自我更新和分化重建了远端前列腺内腔谱系。Dist-Luminal-C细胞中Pten的缺失导致前列腺上皮内瘤变。
 
研究人员进一步表征了11374个人类前列腺细胞,并证实了h-Luminal-C细胞的存在。这项研究提供了对前列腺谱系层次的见解,将Dist-Luminal-C细胞鉴定为内陷尖端中的管腔祖细胞群,并提出了前列腺癌的潜在细胞起源之一。
 
据了解,前列腺干细胞/祖细胞的鉴定和前列腺上皮细胞谱系层次的表征对于理解前列腺癌的发生至关重要。
 
附:英文原文

Title: Single-cell transcriptomics identifies a distinct luminal progenitor cell type in distal prostate invagination tips

Author: Wangxin Guo, Lin Li, Juan He, Zhuang Liu, Ming Han, Fei Li, Xinyi Xia, Xiaoyu Zhang, Yao Zhu, Yu Wei, Yunguang Li, Rebiguli Aji, Hao Dai, Hui Wei, Chunfeng Li, Yu Chen, Luonan Chen, Dong Gao

Issue&Volume: 2020-08-17

Abstract: The identification of prostate stem/progenitor cells and characterization of the prostate epithelial cell lineage hierarchy are critical for understanding prostate cancer initiation. Here, we characterized 35,129 cells from mouse prostates, and identified a unique luminal cell type (termed type C luminal cell (Luminal-C)) marked by Tacstd2, Ck4 and Psca expression. Luminal-C cells located at the distal prostate invagination tips (termed Dist-Luminal-C) exhibited greater capacity for organoid formation in vitro and prostate epithelial duct regeneration in vivo. Lineage tracing of Luminal-C cells indicated that Dist-Luminal-C cells reconstituted distal prostate luminal lineages through self-renewal and differentiation. Deletion of Pten in Dist-Luminal-C cells resulted in prostatic intraepithelial neoplasia. We further characterized 11,374 human prostate cells and confirmed the existence of h-Luminal-C cells. Our study provides insights into the prostate lineage hierarchy, identifies Dist-Luminal-C cells as the luminal progenitor cell population in invagination tips and suggests one of the potential cellular origins of prostate cancer. Single-cell RNA-seq and in vivo lineage tracing identify unique luminal progenitors in the mouse prostate that can contribute to regeneration and oncogenesis. Single-cell RNA-seq analysis of human prostate identifies a similar cell population.

DOI: 10.1038/s41588-020-0642-1

Source: https://www.nature.com/articles/s41588-020-0642-1

期刊信息

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:25.455
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex