近日,中国科学院上海生化与细胞研究所高栋等研究人员合作,利用单细胞转录组学在远端前列腺内陷尖端中发现不同的腔祖细胞类型。这一研究成果于2020年8月17日在线发表在《自然—遗传学》上。
Title: Single-cell transcriptomics identifies a distinct luminal progenitor cell type in distal prostate invagination tips
Author: Wangxin Guo, Lin Li, Juan He, Zhuang Liu, Ming Han, Fei Li, Xinyi Xia, Xiaoyu Zhang, Yao Zhu, Yu Wei, Yunguang Li, Rebiguli Aji, Hao Dai, Hui Wei, Chunfeng Li, Yu Chen, Luonan Chen, Dong Gao
Issue&Volume: 2020-08-17
Abstract: The identification of prostate stem/progenitor cells and characterization of the prostate epithelial cell lineage hierarchy are critical for understanding prostate cancer initiation. Here, we characterized 35,129 cells from mouse prostates, and identified a unique luminal cell type (termed type C luminal cell (Luminal-C)) marked by Tacstd2, Ck4 and Psca expression. Luminal-C cells located at the distal prostate invagination tips (termed Dist-Luminal-C) exhibited greater capacity for organoid formation in vitro and prostate epithelial duct regeneration in vivo. Lineage tracing of Luminal-C cells indicated that Dist-Luminal-C cells reconstituted distal prostate luminal lineages through self-renewal and differentiation. Deletion of Pten in Dist-Luminal-C cells resulted in prostatic intraepithelial neoplasia. We further characterized 11,374 human prostate cells and confirmed the existence of h-Luminal-C cells. Our study provides insights into the prostate lineage hierarchy, identifies Dist-Luminal-C cells as the luminal progenitor cell population in invagination tips and suggests one of the potential cellular origins of prostate cancer. Single-cell RNA-seq and in vivo lineage tracing identify unique luminal progenitors in the mouse prostate that can contribute to regeneration and oncogenesis. Single-cell RNA-seq analysis of human prostate identifies a similar cell population.
DOI: 10.1038/s41588-020-0642-1
Source: https://www.nature.com/articles/s41588-020-0642-1
Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:25.455
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex