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NPR1调控植物免疫反应过程中的细胞存活
作者:小柯机器人 发布时间:2020/8/18 16:45:19

美国杜克大学Xinnian Dong研究组取得最新进展。他们发现系统获得性抗性的激活剂(NPR1)聚合物的形成促进植物免疫反应过程中的细胞存活。这一研究成果发表在2020年8月17日出版的《细胞》杂志上。

他们显示NPR1通过靶向泛素化底物和通过水杨酸诱导的NPR1聚合物(SINCs)的形成来促进细胞存活。SINC富含应激反应蛋白,包括核苷酸结合的富含亮氨酸的重复免疫受体,氧化和DNA损伤反应蛋白以及蛋白质质量控制机制。NPR1-Cullin 3 E3连接酶复合物的形成需要NPR1转变成聚合物,即以泛素化SINC定位的底物,例如EDS1和特定的WRKY转录因子,并在病原体效应物触发的免疫(ETI)期间促进细胞存活。

他们对SINC的分析表明,NPR1通过调节这种准细胞器中的多个胁迫响应过程,而集中整合到植物免疫中的细胞死亡或存活决定中。

研究人员表示,在植物中,ETI通常会导致程序性细胞死亡,这受NPR1限制。但是,尚不清楚NPR1能够促进防御和限制细胞死亡的生化活性。

附:英文原文

Title: Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response

Author: Raul Zavaliev, Rajinikanth Mohan, Tianyuan Chen, Xinnian Dong

Issue&Volume: 2020-08-17

Abstract: In plants, pathogen effector-triggered immunity (ETI) often leads to programmed celldeath, which is restricted by NPR1, an activator of systemic acquired resistance.However, the biochemical activities of NPR1 enabling it to promote defense and restrictcell death remain unclear. Here we show that NPR1 promotes cell survival by targetingsubstrates for ubiquitination and degradation through formation of salicylic acid-inducedNPR1 condensates (SINCs). SINCs are enriched with stress response proteins, includingnucleotide-binding leucine-rich repeat immune receptors, oxidative and DNA damageresponse proteins, and protein quality control machineries. Transition of NPR1 intocondensates is required for formation of the NPR1-Cullin 3 E3 ligase complex to ubiquitinateSINC-localized substrates, such as EDS1 and specific WRKY transcription factors, andpromote cell survival during ETI. Our analysis of SINCs suggests that NPR1 is centrallyintegrated into the cell death or survival decisions in plant immunity by modulatingmultiple stress-responsive processes in this quasi-organelle.

DOI: 10.1016/j.cell.2020.07.016

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30881-3

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/