近日，中国科学院神经科学研究所王凯团队利用共聚焦光场显微镜在小鼠和斑马鱼脑中实现高速体积动态成像。相关论文于2020年8月10日在线发表于《自然—生物技术》。

Title: Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy

Author: Zhenkun Zhang, Lu Bai, Lin Cong, Peng Yu, Tianlei Zhang, Wanzhuo Shi, Funing Li, Jiulin Du, Kai Wang

Issue&Volume: 2020-08-10

Abstract: A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers. It uses a generalized confocal detection scheme that selectively collects fluorescent signals from the in-focus volume and provides optical sectioning capability to improve imaging resolution and sensitivity in thick tissues. We demonstrate recording of whole-brain calcium transients in freely swimming zebrafish larvae and observe behaviorally correlated activities in single neurons during prey capture. Furthermore, in the mouse brain, we detect neural activities at depths of up to 370μm and track blood cells at 70Hz over a volume of diameter 800μm ×thickness 150μm and depth of up to 600μm.

DOI: 10.1038/s41587-020-0628-7

Source: https://www.nature.com/articles/s41587-020-0628-7