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单细胞测序揭示COVID-19和流感患者的不同免疫应答
作者:小柯机器人 发布时间:2020/7/21 9:42:54

近日,中国疾病预防控制中心William J. Liu等研究人员合作利用外周血单核细胞的单细胞测序,揭示了COVID-19和流感患者的独特免疫应答情况。相关论文于2020年7月19日在线发表在《免疫》杂志上。

研究人员报告了在COVID-19和A型流感病毒(IAV)感染的患者中,纵向收集的外周血单个核细胞(PBMC)的单细胞转录图谱。研究人员观察到在COVID-19和IAV患者中浆细胞增加,并且在COVID-19患者中XAF1、TNF-和FAS诱导的T细胞凋亡增加。

进一步的分析显示,COVID-19(STAT1和IRF3)与IAV(STAT3和NFκB)患者相比,激活的信号通路不同,并且关键因子的表达存在实质性差异。这些因素包括在COVID-19患者中IL6R和IL6ST表达的相对增加,但与IAV患者相比,IL-6浓度的升高类似,这与临床观察到的COVID-19患者中促炎性细胞因子增加一致。因此,研究人员提供了PBMC的表达概况,并揭示了COVID-19和IAV患者的独特免疫应答途径。

COVID-19是一种严重的传染病,目前是全球健康威胁。但是,与其他传染病相比,人们对其标志物的了解还很少。

附:英文原文

Title: Single-cell sequencing of peripheral blood mononuclear cells reveals distinct immune response landscapes of COVID-19 and influenza patients

Author: Linnan Zhu, Penghui Yang, Yingze Zhao, Zhenkun Zhuang, Zhifeng Wang, Rui Song, Jie Zhang, Chuanyu Liu, Qianqian Gao, Qumiao Xu, Xiaoyu Wei, Hai-Xi Sun, Beiwei Ye, Yanan Wu, Ning Zhang, Guanglin Lei, Linxiang Yu, Jin Yan, Guanghao Diao, Fanping Meng, Changqing Bai, Panyong Mao, Yeya Yu, Mingyue Wang, Yue Yuan, Qiuting Deng, Ziyi Li, Yunting Huang, Guohai Hu, Yang Liu, Xiaoqian Wang, Ziqian Xu, Peipei Liu, Yuhai Bi, Yi Shi, Shaogeng Zhang, Zhihai Chen, Jian Wang, Xun Xu, Guizhen Wu, Fusheng Wang, George F. Gao, Longqi Liu, William J. Liu

Issue&Volume: 2020-07-19

Abstract: COVID-19 is a severe infectious disease that is a current global health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cells (PBMCs) in both COVID-19 and influenza A virus (IAV)-infected patients. We observed increase of plasma cells in both COVID-19 and IAV patients, and XAF1-, TNF- and FAS-induced T cell apoptosis in COVID-19 patients. Further analyses revealed distinct signaling pathways activated in COVID-19 (STAT1 and IRF3) vs. IAV (STAT3 and NFκB) patients and substantial differences in the expression of key factors. These factors include relatively increase of IL6R and IL6ST expression in COVID-19 patients, but similarly increased IL-6 concentrations compared to IAV patients, supporting the clinical observations of increased pro-inflammatory cytokines in COVID-19 patients. Thus, we provide the landscape of PBMCs and unveil distinct immune response pathways in COVID-19 and IAV patients.

DOI: 10.1016/j.immuni.2020.07.009

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30316-2

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新if:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx