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研究揭示病毒感染时浆细胞样树突状细胞在体内的激活轨迹
作者:小柯机器人 发布时间:2020/7/21 14:37:55

法国艾克斯-马赛大学Elena Tomasello研究团队经过努力,揭示了病毒感染期时体内浆细胞样树突状细胞(pDC)的激活轨迹。相关论文于2020年70日发表于《自然-免疫学》杂志。

研究人员通过将Ifnb1报告基因小鼠与流式细胞仪、单细胞RNA测序、共聚焦显微镜和相关的CD4 T细胞活化分析相结合的方法,深入分析了病毒感染小鼠pDC的活化状态。研究发现I型干扰素(IFN-I)的产生和T细胞激活是由相同的pDC进行的,但其发生时间和顺序依次出现在不同的微解剖位置。

此外,研究人员还发现pDC对IFN-I产生的调控在早期就被其下调的白血病抑制因子受体所标记,并被细胞内肿瘤坏死因子信号所促进。该研究提出了一种新的模型,用于说明在病毒感染过程中如何以时间和空间紧密协调的方式赋予单个pDC在体内发挥不同的功能。

据介绍,pDC是IFN-I的主要来源。病毒感染时pDC在体内是否发挥其他功能尚存争议,因此,需要更多的研究来了解其编排。

附:英文原文

Title: The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection

Author: Abdenour Abbas, Thien-Phong Vu Manh, Michael Valente, Nils Collinet, Noudjoud Attaf, Chuang Dong, Karima Naciri, Rabie Chelbi, Geoffray Brelurut, Inaki Cervera-Marzal, Benjamin Rauwel, Jean-Luc Davignon, Gilles Bessou, Morgane Thomas-Chollier, Denis Thieffry, Alexandra-Chlo Villani, Pierre Milpied, Marc Dalod, Elena Tomasello

Issue&Volume: 2020-07-20

Abstract: Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial, and more studies are needed to understand their orchestration. In the present study, we characterize in depth and link pDC activation states in animals infected by mouse cytomegalovirus by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but these occurred sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of leukemia inhibitory factor receptor and was promoted by cell-intrinsic tumor necrosis factor signaling. We propose a new model for how individual pDCs are endowed to exert different functions in vivo during a viral infection, in a manner tightly orchestrated in time and space. Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). Dalod and colleagues show that IFN-I production and T cell activation were performed by the same pDC, but these occurred sequentially and in different micro-anatomical locations during virus infection.

DOI: 10.1038/s41590-020-0731-4

Source: https://www.nature.com/articles/s41590-020-0731-4

期刊信息

Nature Immunology:《自然—免疫学》,创刊于2000年。隶属于施普林格·自然出版集团,最新IF:23.53
官方网址:https://www.nature.com/ni/
投稿链接:https://mts-ni.nature.com/cgi-bin/main.plex