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综述:基于蛋白酶的细胞生物学传感和控制前沿方法
作者:小柯机器人 发布时间:2020/7/16 16:07:43

斯坦福大学Michael Z. Lin研究小组发表综述,回顾了现有的基于蛋白酶的传感和控制细胞生物学的前沿方法。 该文发表在2020年7月13日出版的《自然—方法学》上。

该综述全面回顾了基于蛋白酶的方法的发展及其在报告和控制真核生物细胞功能上的运用。该综述主要介绍用于细胞生物学上传感和控制蛋白功能的基于蛋白酶的工具。

特定序列蛋白酶已被证明是报告或控制细胞功能的工具的多样构建模块。已报告方法通过链接蛋白酶活性到特定生化信号,并使用能够响应如光或化学物质的内源性输入信号的工程蛋白酶进行控制。反过来,由于蛋白酶本身的功能就是释放,激活或破坏蛋白质,并由于蛋白酶水解的不可逆性能够引发持续的下游效应,蛋白酶有其固有的控制能力。因此,基于蛋白酶的合成“电路”可以被创建并具有如报告细胞信号,调控蛋白表达,控制病毒复制和检测癌症的多样化用途。

附:英文原文

Title: On the cutting edge: protease-based methods for sensing and controlling cell biology

Author: H. Kay Chung, Michael Z. Lin

Issue&Volume: 2020-07-13

Abstract: Sequence-specific proteases have proven to be versatile building blocks for tools that report or control cellular function. Reporting methods link protease activity to biochemical signals, whereas control methods rely on engineering proteases to respond to exogenous inputs such as light or chemicals. In turn, proteases have inherent control abilities, as their native functions are to release, activate or destroy proteins by cleavage, with the irreversibility of proteolysis allowing sustained downstream effects. As a result, protease-based synthetic circuits have been created for diverse uses such as reporting cellular signaling, tuning protein expression, controlling viral replication and detecting cancer states. Here, we comprehensively review the development and application of protease-based methods for reporting and controlling cellular function in eukaryotes. This Review focuses on protease-based tools for sensing and controlling protein function in cell biology.

DOI: 10.1038/s41592-020-0891-z

Source: https://www.nature.com/articles/s41592-020-0891-z

期刊信息

Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex