美国范德比尔特大学医学中心James E. Crowe、Robert H. Carnahan等研究人员合作，实现了对新冠病毒（SARS-CoV-2）突刺蛋白人源单克隆抗体的快速分离与鉴定。这一研究成果于2020年7月10日在线发表在《自然—医学》上。

研究人员使用了快速抗体发现平台来分离数百种靶向SARS-CoV-2突刺（S）蛋白的人类单克隆抗体（mAb）。基于它们与S蛋白亚结构域的反应性以及与SARS-CoV的交叉反应性，研究人员将这些单克隆抗体分为五个主要类别。

这些单克隆抗体中有许多会抑制真正的SARS-CoV-2病毒的感染，大多数中和的单克隆抗体会识别S的受体结合域（RBD）。这项工作确定了SARS-CoV-2 S上的易感位点，并证明了这一抗体发现平台的速度和稳定性 。

据了解，抗体是大多数RNA病毒免疫力的主要决定因素，并有望减少流行病期间的感染或疾病。

附：英文原文

Title: Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

Author: Seth J. Zost, Pavlo Gilchuk, Rita E. Chen, James Brett Case, Joseph X. Reidy, Andrew Trivette, Rachel S. Nargi, Rachel E. Sutton, Naveenchandra Suryadevara, Elaine C. Chen, Elad Binshtein, Swathi Shrihari, Mario Ostrowski, Helen Y. Chu, Jonathan E. Didier, Keith W. MacRenaris, Taylor Jones, Samuel Day, Luke Myers, F. Eun-Hyung Lee, Doan C. Nguyen, Ignacio Sanz, David R. Martinez, Paul W. Rothlauf, Louis-Marie Bloyet, Sean P. J. Whelan, Ralph S. Baric, Larissa B. Thackray, Michael S. Diamond, Robert H. Carnahan, James E. Crowe

Issue&Volume: 2020-07-10

Abstract: Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms. 

DOI: 10.1038/s41591-020-0998-x

Source: https://www.nature.com/articles/s41591-020-0998-x