美国纽约大学Boris Reizis、爱因斯坦医学院David R. Fooksman等研究人员合作，利用活体内成像揭示了骨髓（BM）微环境中成年造血干细胞的运动性。这一研究成果于2020年6月25日在线发表在《细胞—干细胞》上。
Title: Intravital Imaging Reveals Motility of Adult Hematopoietic Stem Cells in the Bone Marrow Niche
Author: Samik Upadhaya, Oleg Krichevsky, Ilseyar Akhmetzyanova, Catherine M. Sawai, David R. Fooksman, Boris Reizis
Abstract: Adult mammalian hematopoietic stem cells (HSCs) reside in the bone marrow (BM) butcan be mobilized into blood for use in transplantation. HSCs interact with BM nichecells that produce growth factor c-Kit ligand (Kitl/SCF) and chemokine CXCL12, andwere thought to be static and sessile. We used two-photon laser scanning microscopyto visualize genetically labeled HSCs in the BM of live mice for several hours. Themajority of HSCs showed a dynamic non-spherical morphology and significant motility,undergoing slow processive motion interrupted by short stretches of confined motion.HSCs moved in the perivascular space and showed intermittent close contacts with SCF-expressingperivascular stromal cells. In contrast, mobilization-inducing blockade of CXCL12receptor CXCR4 and integrins rapidly abrogated HSC motility and shape dynamics inreal time. Our results reveal an unexpectedly dynamic nature of HSC residence in theBM and interaction with the SCF+ stromal niche, which is disrupted during HSC mobilization.
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：21.464