新加坡南洋理工大学Lai Guan Ng、Immanuel Kwok等研究人员合作，利用组合单细胞分析发现粒细胞-单核细胞前体（CMP）的异质性并揭示出早期的单潜能中性粒细胞前体。相关论文于2020年6月23日在线发表于《免疫》。
Title: Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor
Author: Immanuel Kwok, Etienne Becht, Yu Xia, Melissa Ng, Ye Chean Teh, Leonard Tan, Maximilien Evrard, Jackson L.Y. Li, Hoa T.N. Tran, Yingrou Tan, Dehua Liu, Archita Mishra, Ka Hang Liong, Keith Leong, Yuning Zhang, Andre Olsson, Chinmay Kumar Mantri, Pavithra Shyamsunder, Zhaoyuan Liu, Cecile Piot, Charles-Antoine Dutertre, Hui Cheng, Sudipto Bari, Nicholas Ang, Subhra K. Biswas, H. Philip Koeffler, Hong Liang Tey, Anis Larbi, I-Hsin Su, Bernett Lee, Ashley St. John, Jerry K.Y. Chan, William Y.K. Hwang, Jinmiao Chen, Nathan Salomonis, Shu Zhen Chong, H. Leighton Grimes, Bing Liu, Andrés Hidalgo, Evan W. Newell, Tao Cheng, Florent Ginhoux, Lai Guan Ng
Abstract: Granulocyte-monocyte progenitors (GMPs) have been previously defined for their potentialto generate various myeloid progenies such as neutrophils and monocytes. Althoughstudies have proposed lineage heterogeneity within GMPs, it is unclear if committedprogenitors already exist among these progenitors and how they may behave differentlyduring inflammation. By combining single-cell transcriptomic and proteomic analyses,we identified the early committed progenitor within the GMPs responsible for the strictproduction of neutrophils, which we designate as proNeu1. Our dissection of the GMPhierarchy led us to further identify a previously unknown intermediate proNeu2 population.Similar populations could be detected in human samples. proNeu1s, but not proNeu2s,selectively expanded during the early phase of sepsis at the expense of monocytes.Collectively, our findings help shape the neutrophil maturation trajectory roadmapand challenge the current definition of GMPs.