美国康奈尔大学Shuibing Chen、Robert E. Schwartz、Todd Evans等研究人员合作开发出一个基于人类多能干细胞的平台，可用于建立新冠病毒（SARS-CoV-2）感染模型。该研究于2020年6月19日在线发表于《细胞—干细胞》。
Title: A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
Author: Liuliu Yang, Yuling Han, Benjamin E. Nilsson-Payant, Vikas Gupta, Pengfei Wang, Xiaohua Duan, Xuming Tang, Jiajun Zhu, Zeping Zhao, Fabrice Jaffré, Tuo Zhang, Tae Wan Kim, Oliver Harschnitz, David Redmond, Sean Houghton, Chengyang Liu, Ali Naji, Gabriele Ciceri, Sudha Guttikonda, Yaron Bram, Duc-Huy T. Nguyen, Michele Cioffi, Vasuretha Chandar, Daisy A. Hoagland, Yaoxing Huang, Jenny Xiang, Hui Wang, David Lyden, Alain Borczuk, Huanhuan Joyce Chen, Lorenz Studer, Fong Cheng Pan, David D. Ho, Benjamin R. tenOever, Todd Evans, Robert E. Schwartz, Shuibing Chen
Abstract: SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from human pluripotent stem cells (hPSCs). A Spike-enabled pseudo-entry virus infects pancreatic endocrine cells, liver organoids, cardiomyocytes, and dopaminergic neurons. Recent clinical studies show a strong association with COVID-19 and diabetes. We find that human pancreatic beta cells and liver organoids are highly permissive to SARS-CoV-2 infection, further validated using adult primary human islets and adult hepatocyte and cholangiocyte organoids. SARS-CoV-2 infection caused striking expression of chemokines, as also seen in primary human COVID-19 pulmonary autopsy samples. hPSC-derived cells/organoids provide valuable models for understanding the cellular responses of human tissues to SARS-CoV-2 infection and for disease modeling of COVID-19.
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：21.464