英国伦敦大学学院R. Angus Silver研究小组开发出能够对活动动物进行实时3D运动校正的双光子成像技术。该项研究成果于2020年6月1日在线发表在《自然—方法学》杂志上。
Title: Real-time 3D movement correction for two-photon imaging in behaving animals
Author: Victoria A. Griffiths, Antoine M. Valera, Joanna YN. Lau, Hana Ro, Thomas J. Younts, Bris Marin, Chiara Baragli, Diccon Coyle, Geoffrey J. Evans, George Konstantinou, Theo Koimtzis, K. M. Naga Srinivas Nadella, Sameer A. Punde, Paul A. Kirkby, Isaac H. Bianco, R. Angus Silver
Abstract: Two-photon microscopy is widely used to investigate brain function across multiple spatial scales. However, measurements of neural activity are compromised by brain movement in behaving animals. Brain motion-induced artifacts are typically corrected using post hoc processing of two-dimensional images, but this approach is slow and does not correct for axial movements. Moreover, the deleterious effects of brain movement on high-speed imaging of small regions of interest and photostimulation cannot be corrected post hoc. To address this problem, we combined random-access three-dimensional (3D) laser scanning using an acousto-optic lens and rapid closed-loop field programmable gate array processing to track 3D brain movement and correct motion artifacts in real time at up to 1kHz. Our recordings from synapses, dendrites and large neuronal populations in behaving mice and zebrafish demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision.