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脑肿瘤微环境图谱揭示疾病特异性免疫细胞改变
作者:小柯机器人 发布时间:2020/5/30 16:34:32

瑞士洛桑大学Johanna A. Joyce研究小组通过绘制脑肿瘤微环境图谱揭示出疾病特异性免疫细胞改变。这一研究成果于2020年5月28日在线发表于《细胞》。

研究人员通过流式细胞仪、RNA测序、蛋白质阵列、培养测定法和空间组织表征全面分析了脑肿瘤微环境(TME)。这揭示了免疫细胞的疾病特异性富集,在组织驻留小胶质细胞、浸润单核细胞衍生的巨噬细胞、嗜中性粒细胞和T细胞的比例丰度方面存在明显差异。
 
这些综合分析还发现了脑恶性肿瘤中多方面的免疫细胞活化,涉及到汇集的转录轨迹,同时还能维持疾病和细胞类型特异性程序。对开发靶向脑恶性肿瘤的TME疗法,这些免疫资源为克服TME并治疗肿瘤提供了新的见解。
 
据了解,脑恶性肿瘤包括一系列原发性和转移性癌症,包括低度和高度神经胶质瘤以及源自多种颅外肿瘤的脑转移瘤。人们对脑肿瘤微环境(TME)的了解仍然有限,并且尚不清楚它是由原发性疾病还是转移性疾病导致。
 
附:英文原文

Title: Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells

Author: Florian Klemm, Roeltje R. Maas, Robert L. Bowman, Mara Kornete, Klara Soukup, Sina Nassiri, Jean-Philippe Brouland, Christine A. Iacobuzio-Donahue, Cameron Brennan, Viviane Tabar, Philip H. Gutin, Roy T. Daniel, Monika E. Hegi, Johanna A. Joyce

Issue&Volume: 2020-05-28

Abstract: Brain malignancies encompass a range of primary and metastatic cancers, includinglow-grade and high-grade gliomas and brain metastases (BrMs) originating from diverseextracranial tumors. Our understanding of the brain tumor microenvironment (TME) remainslimited, and it is unknown whether it is sculpted differentially by primary versusmetastatic disease. We therefore comprehensively analyzed the brain TME landscapevia flow cytometry, RNA sequencing, protein arrays, culture assays, and spatial tissuecharacterization. This revealed disease-specific enrichment of immune cells with pronounceddifferences in proportional abundance of tissue-resident microglia, infiltrating monocyte-derivedmacrophages, neutrophils, and T cells. These integrated analyses also uncovered multifacetedimmune cell activation within brain malignancies entailing converging transcriptionaltrajectories while maintaining disease- and cell-type-specific programs. Given theinterest in developing TME-targeted therapies for brain malignancies, this comprehensiveresource of the immune landscape offers insights into possible strategies to overcometumor-supporting TME properties and instead harness the TME to fight cancer.

DOI: 10.1016/j.cell.2020.05.007

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30569-9

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/