德国凯撒研究中心Jason N. D. Kerr等研究人员开发出新型三光子头戴式显微镜，可用于自由活动大鼠中的深层皮质成像。相关论文于2020年5月4日在线发表在《自然—方法学》杂志上。

Title: Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

Author: Alexandr Klioutchnikov, Damian J. Wallace, Michael H. Frosz, Richard Zeltner, Juergen Sawinski, Verena Pawlak, Kay-Michael Voit, Philip St. J. Russell, Jason N. D. Kerr

Issue&Volume: 2020-05-04

Abstract: We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1h in freely moving rats performing a range of behaviors.

DOI: 10.1038/s41592-020-0817-9

Source: https://www.nature.com/articles/s41592-020-0817-9