英国南安普顿大学Max Crispin团队报道了新冠病毒(SARS-CoV-2 )突刺蛋白的位点特异性聚糖分析。相关论文于2020年5月4日在线发表在《科学》杂志上。
Title: Site-specific glycan analysis of the SARS-CoV-2 spike
Author: Yasunori Watanabe, Joel D. Allen, Daniel Wrapp, Jason S. McLellan, Max Crispin
Issue&Volume: 2020/05/04
Abstract: Abstract The emergence of the betacoronavirus, SARS-CoV-2, the causative agent of COVID-19, represents a significant threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design.
DOI: 10.1126/science.abb9983
Source: https://science.sciencemag.org/content/early/2020/05/01/science.abb9983