美国哈佛大学Hao Wu、Judy Lieberman、Xing Liu等研究人员合作发现，FDA批准的双硫仑通过阻断gasdermin D（GSDMD）膜孔的形成来抑制焦亡。这项研究成果于2020年5月4日在线发表在《自然—免疫学》杂志上。

Title: FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation

Author: Jun Jacob Hu, Xing Liu, Shiyu Xia, Zhibin Zhang, Ying Zhang, Jingxia Zhao, Jianbin Ruan, Xuemei Luo, Xiwen Lou, Yang Bai, Junhong Wang, L. Robert Hollingsworth, Venkat Giri Magupalli, Li Zhao, Hongbo R. Luo, Justin Kim, Judy Lieberman, Hao Wu

Issue&Volume: 2020-05-04

Abstract: Cytosolic sensing of pathogens and damage by myeloid and barrier epithelial cells assembles large complexes called inflammasomes, which activate inflammatory caspases to process cytokines (IL-1β) and gasdermin D (GSDMD). Cleaved GSDMD forms membrane pores, leading to cytokine release and inflammatory cell death (pyroptosis). Inhibiting GSDMD is an attractive strategy to curb inflammation. Here we identify disulfiram, a drug for treating alcohol addiction, as an inhibitor of pore formation by GSDMD but not other members of the GSDM family. Disulfiram blocks pyroptosis and cytokine release in cells and lipopolysaccharide-induced septic death in mice. At nanomolar concentration, disulfiram covalently modifies human/mouse Cys191/Cys192 in GSDMD to block pore formation. Disulfiram still allows IL-1β and GSDMD processing, but abrogates pore formation, thereby preventing IL-1β release and pyroptosis. The role of disulfiram in inhibiting GSDMD provides new therapeutic indications for repurposing this safe drug to counteract inflammation, which contributes to many human diseases.

DOI: 10.1038/s41590-020-0669-6

Source: https://www.nature.com/articles/s41590-020-0669-6